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Combinatorial Chemistry & High Throughput Screening

Editor-in-Chief

ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

Research Article

Salivary Antioxidant and Oxidative Stress Marker Levels in HIV-Positive Individuals

Author(s): Samaneh Vaziri Amjad, Poorandokht Davoodi, Mohammad Taghi Goodarzi, Hamidreza Abdolsamadi, Jalal Poorolajal, Shahryar Parsa, Darvish Paydari and Fatemeh Ahmadi-Motamayel*

Volume 22, Issue 1, 2019

Page: [59 - 64] Pages: 6

DOI: 10.2174/1386207322666190306144629

Price: $65

Abstract

Background: HIV infections are a worldwide health problem. HIV infection reduces CD4+ cell counts. Oxidative stress might play an important role in the stimulation of virus replication and immunodeficiency. Saliva might be the first line of defense against oxidative stress.

Objective: The aim of this study was to evaluate the oxidative stress marker and antioxidant levels of saliva in HIV-infected patients by measuring total antioxidant capacity and malondialdehyde level.

Methods: A total of 49 HIV-positive patients and 49 healthy HIV-negative individuals were randomly selected. All the patients were clinically examined. Five mL of unstimulated whole saliva was collected and evaluated by spectrophotometric assay. Data were analyzed with STATA 11.

Results: Mean ages of the case and control groups were 28 and 33 years, respectively. Salivary malondialdehyde levels were significantly higher in the HIV-positive group (3.68±2.26) compared to the healthy control group (2.79±1.91). Levels of salivary total antioxidant capacity were significantly lower in the HIV-positive group (0.20± 0.09) compared to the control group (0.27±0.10).

Conclusion: The antioxidant defense system in HIV-positive individuals was low and oxidative stress was high in this population. Saliva might be used as a diagnostic tool for antioxidant changes in HIV-positive patients in the future. There were changes in salivary antioxidant defense system and oxidative stress in HIV-positive individuals. Antioxidant supplements might help local salivary and general health statuses.

Keywords: HIV, antioxidants, oxidative stress, saliva, biomarkers, data analysis.

[1]
Bodhade, A.S.; Ganvir, S.M.; Hazarey, V.K. Oral manifestations of HIV infection and their correlation with CD4 count. J. Oral Sci., 2011, 53(2), 203-211.
[2]
Davoodi, P.; Hamian, M.; Nourbaksh, R.; Motamayel, F.A. Oral Manifestations Related To CD4 Lymphocyte Count in HIV-Positive Patients. JODDD, 2010, 4(4), 115-119.
[3]
Dickinson, B.C.; Chang, C.J. Chemistry and biology of reactive oxygen species in signaling or stress responses. Nat. Chem. Biol., 2011, 7(8), 504-511.
[4]
Higgs, M.R.; Chouteau, P.; Lerat, H. ‘Liver let die’: Oxidative DNA damage and hepatotropic viruses. J. Gen. Virol., 2014, 95(Pt 5), 991-1004.
[5]
Couret, J.; Chang, T.L. Reactive oxygen species in HIV infection. EC Microbiol., 2016, 3(6), 597-604.
[6]
Kalinowska, M.; Bazdar, D.A.; Lederman, M.M.; Funderburg, N.; Sieg, S.F. Decreased IL-7 responsiveness is related to oxidative stress in HIV disease. PLoS One, 2013, 8(3), e58764.
[7]
Mebrat, Y.; Amogne, W.; Mekasha, A.; Gleason, R.L., Jr; Seifu, D. Lipid peroxidation and altered antioxidant profiles with pediatric HIV infection and antiretroviral therapy in Addis Ababa, Ethiopia. J. Trop. Pediatr., 2017, 63(3), 196-202.
[8]
Watanabe, L.M.; Barbosa, F., Junior; Jordao, A.A.; Navarro, A.M. Influence of HIV infection and the use of antiretroviral therapy on selenium and selenomethionine concentrations and antioxidant protection. Nutrition, 2016, 32(11-12), 1238-1242.
[9]
Teto, G.; Kanmogne, G.D.; Torimiro, J.N.; Alemnji, G.; Nguemaim, F.N.; Takou, D.; Nanfack, A.; Tazoacha, A. Lipid peroxidation and total cholesterol in HAART-naive patients infected with circulating recombinant forms of human immunodeficiency virus type-1 in Cameroon. PLoS One, 2013, 8(6), e65126.
[10]
Lan, X.; Cheng, K.; Chandel, N.; Lederman, R.; Jhaveri, A.; Husain, M.; Malhotra, A.; Singhal, P.C. High glucose enhances HIV entry into T cells through upregulation of CXCR4. J. Leukoc. Biol., 2013, 94(4), 769-777.
[11]
Parsons, M.; Campa, A.; Lai, S.; Li, Y.; Martinez, J.D.; Murillo, J.; Greer, P.; Martinez, S.S.; Baum, M.K. Effect of GSTM1-Polymorphism on disease progression and oxidative stress in HIV infection: Modulation by HIV/HCV co-infection and alcohol consumption. J. AIDS Clin. Res., 2013, 4(9), 10002337.
[12]
Deshmane, S.L.; Mukerjee, R.; Fan, S.; Del Valle, L.; Michiels, C.; Sweet, T.; Rom, I.; Khalili, K.; Rappaport, J.; Amini, S.; Sawaya, B.E. Activation of the oxidative stress pathway by HIV-1 Vpr leads to induction of hypoxia-inducible factor 1alpha expression. J. Biol. Chem., 2009, 284(17), 11364-11373.
[13]
Ivanov, A.V.; Valuev-Elliston, V.T. Oxidative Stress during HIV infection: Mechanisms and consequences. Omid. Med. Cell Longer., 2016, 2016, 8910396.
[14]
Javaid, M.A.; Ahmed, A.S.; Durand, R.; Tran, S.D. Saliva as a diagnostic tool for oral and systemic diseases. J. Oral Biol. Craniofac. Res., 2016, 6(1), 66-75.
[15]
Bartosz, G. Total antioxidant capacity. Adv. Clin. Chem., 2003, 37, 219-292.
[16]
Ahmadi-Motamayel, F.; Amjad, S.V.; Goodarzi, M.T.; Poorolajal, J. Evaluation of salivary uric acid and pH in human immunodeficiency virus infected patients: A historical cohort study. Infect. Disord. Drug Targets, 2018, 18(1), 35-40.
[17]
Ahmadi-Motamayel, F.; Falsafi, P.; Goodarzi, M.T.; Poorolajal, J. Comparison of salivary ph, buffering capacity and alkaline phosphatase in smokers and healthy Non-Smokers: Retrospective cohort study. Sultan Qaboos Univ. Med. J., 2016, 16(3), e317-e321.
[18]
Ahmadi-Motamayel, F.; Vaziri-Amjad, S.; Goodarzi, M.T.; Poorolajal, J. Evaluation of salivary vitamin C and catalase in HIV positive and healthy HIV negative control group. Infect. Disord. Drug Targets, 2017, 17(2), 101-105.
[19]
Ahmadi-Motamayel, F.; Vaziri-Amjad, S.; Goodarzi, M.T.; Samie, L.; Poorolajal, J. Evaluation of Salivary melatonin levels in HIV-positive patients: A historical cohort study. Rev. Recent Clin. Trials, 2017, 12(3), 168-173.
[20]
Ahmadi-Motamayel, F.; Goodarzi, M.T.; Hendi, S.S.; Kasraei, S.; Moghimbeigi, A. Total antioxidant capacity of saliva and dental caries. Med. Oral Patol. Oral Cir. Bucal, 2013, 18(4), e553-e556.
[21]
Canakci, C.F.; Cicek, Y.; Yildirim, A.; Sezer, U.; Canakci, V. Increased levels of 8-hydroxydeoxyguanosine and malondialdehyde and its relationship with antioxidant enzymes in saliva of periodontitis patients. Eur. J. Dent., 2009, 3(2), 100-106.
[22]
Buczko, P.; Zalewska, A.; Szarmach, I. Saliva and oxidative stress in oral cavity and in some systemic disorders. J. Physiol. Pharmacol., 2015, 66(1), 3-9.
[23]
Navazesh, M. Methods for collecting saliva. Ann. N. Y. Acad. Sci., 1993, 694, 72-77.
[24]
Rai, B.; Kharb, S.; Jain, R.; Anand, S. Salivary lipid peroxidation product malonaldehyde in various dental diseases. World J. Med. Sci., 2006, 1, 100-101.
[25]
Riviere, G.R.; Papagiannoulis, L. Antibodies to indigenous and laboratory strains of Streptococcus mutans in saliva from children with dental caries and from caries-free children. Pediatr. Dent., 1987, 9(3), 216-220.
[26]
Yousaf, M.Z.; Zia, S.; Babar, M.E.; Ashfaq, U.A. The epidemic of HIV/AIDS in developing countries; the current scenario in Pakistan. Virol. J., 2011, 8, 401.
[27]
Al-Rawi, N.H. Oxidative stress, antioxidant status and lipid profile in the saliva of type 2 diabetics. Diab. Vasc. Dis. Res., 2011, 8(1), 22-28.
[28]
Kolesnikova, L.I.; Kolesnikov, S.I.; Darenskaya, M.A.; Grebenkina, L.A.; Timofeeva, E.V.; Leshchenko, O.Y.; Vanteeva, O.A.; Rashidova, M.A. Evaluation of the pro- and antioxidant status of women with HIV or coinfection. Terapevticheskii. Arkh., 2016, 88(11), 17-21.
[29]
Rivera-Rivera, Y.; Garcia, Y.; Toro, V.; Cappas, N.; Lopez, P.; Yamamura, Y.; Rivera-Amill, V. Depression correlates with increased plasma levels of inflammatory cytokines and a dysregulated oxidant/antioxidant balance in HIV-1-infected subjects undergoing antiretroviral therapy. J. Clin. Cell. Immunol., 2014, 5(6), 1000276.
[30]
Padmanabhan, V.; Rai, K.; Hegde, A.M.; Shetty, S. Total antioxidant capacity of saliva in children with HIV. J. Clin. Pediatr. Dent., 2010, 34(4), 347-350.
[31]
Wanchu, A.; Rana, S.V.; Pallikkuth, S.; Sachdeva, R.K. Short communication: oxidative stress in HIV-infected individuals: A cross-sectional study. AIDS Res. Hum. Retroviruses, 2009, 25(12), 1307-1311.
[32]
Ahmadi-Motamayel, F.; Goodarzi, M.T.; Mahdavinezhad, A.; Jamshidi, Z.; Darvishi, M. salivary and serum antioxidant and oxidative stress markers in dental caries. Caries Res., 2018, 52(6), 565-569.

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