Login Register Cart 0
Generic placeholder image

The Natural Products Journal

Editor-in-Chief

ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Verbascum sinaiticum: A Rich Source of Antioxidant Phenylethanoid Glycosides

Author(s): Humaira Yasmeen Gondal*, Roshan Zamir, Muhammad Nisar and Muhammad Iqbal Choudhary

Volume 10, Issue 2, 2020

Page: [158 - 162] Pages: 5

DOI: 10.2174/2210315509666190129160405

Price: $65

Abstract

Background: The genus Verbascum is well documented for its antioxidant potential but Verbascum sinaiticum is comparatively less studied plant. The current study was carried out to search for antioxidant nutraceuticals from this species.

Objective: To explore the antioxidant potential of Verbascum sinaiticum and to identify its active constituents.

Method: The methanolic extract of air-dried aerial part of the Verbascum sinaiticum was partitioned with hexane, chloroform and ethyl acetate. The water-soluble part of ethyl acetate afforded six phenylethanoid glycosides by repeated chromatography over Sephadex LH-20, silica gel and ODS columns. Antioxidant activity of solvent extracts and isolated constituents were evaluated by DPPH, ABTS and FRAP assays.

Results: Six phenylethanoid glycosides was isolated and characterized as Verbascoside, Eukovoside, Martynoside, Jionoside D, Campneoside I and Campneoside II, from the most active fraction.

Conclusion: Verbascum sinaiticum demonstrated prospective antioxidant activity. The watersoluble part of EtOAc (WSEAE) was found the most active extract whereas Verbascoside was identified as the most potent constituent. All isolated compounds exhibited significant antioxidant activity whereas their synergistic effect was found prominent in the parent fraction.

Keywords: Antioxidant, Verbascum sinaiticum, phenolic glycosides, phenylethanoid glycosides, radical scavenging ability, nutraceuticals.

Graphical Abstract

[1]
Robert, E.C. Wildman Handbook of nutraceuticals and functional foods, 2nd ed; CRC Press: Boca Raton, FL, 2006, pp. 1-21.
[2]
Tresserra-Rimbau, A.; Lamuela-Raventos, R.M.; Moreno, J.J. Polyphenols, food and pharma. Current knowledge and directions for future research. Biochem. Pharmacol., 2018, 156, 186-195.
[3]
Nasir, S.; Ali, I. Flora of West Pakistan; Shamim Printing Press: Karachi, Pakistan, 1972.
[4]
Philcox, D.; Ghazanfar, S.A. Scrophulariaceae, Flora of Tropical East Africa, Ghazanfar S. A.; Hepper, F.N; Philcox, D., Ed.; Royal Botanic Gardens: Kew, 2008, pp. 67-91.
[5]
Turker, A.U.; Camper, N.D. Biological activity of common mullein, a medicinal plant. J. Ethnopharmacol., 2002, 82(2-3), 117-125.
[6]
Kyriakopoulou, I.; Magiatis, P.; Skaltsounis, A.L.; Aligiannis, N.; Harvala, C. Samioside, a new phenylethanoid glycoside with free-radical scavenging and antimicrobial activities from Phlomis samia. J. Nat. Prod., 2001, 64(8), 1095-1097.
[7]
Tatli, I.I.; Takamatsu, S.; Khan, I.A.; Akdemir, Z.S. Screening for free radical scavenging and cell aggregation inhibitory activities by secondary metabolites from Turkish Verbascum species. Zeitschrift für Naturforschung C, 2007, 62(9-10), 673-678.
[8]
Mergia, E.; Shibeshi, W.; Terefe, G.; Teklehaymanot, T. Antitrypanosomal activity of Verbascum sinaiticum Benth. (Scrophulariaceae) against Trypanosoma congolense isolates. BMC Complement. Altern. Med., 2016, 16(1), 362.
[9]
Tadeg, H.; Mohammed, E.; Asres, K.; Gebre-Mariam, T. Antimicrobial activities of some selected traditional Ethiopian medicinal plants used in the treatment of skin disorders. J. Ethnopharmacol., 2005, 100(1-2), 168-175.
[10]
Khafagi, I.K.; Dewedar, A. The efficiency of random versus ethno-directed research in the evaluation of Sinai medicinal plants for bioactive compounds. J. Ethnopharmacol., 2000, 71(3), 365-376.
[11]
Talib, W.H.; Mahasneh, A.M. Antiproliferative activity of plant extracts used against cancer in traditional medicine. Scient. Pharmaceut., 2010, 78(1), 33.
[12]
Umer, S.; Asres, K.; Veeresham, C. Hepatoprotective activities of two Ethiopian medicinal plants. Pharm. Boil., 2010, 48(4), 461-468.
[13]
Talib, W.H.; Mahasneh, A.M. Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine. Molecules, 2010, 15(3), 1811-1824.
[14]
Afifi, M.S.; Ahmed, M.M.; Pezzuto, J.M.; Kinghornt, A.D. Cytotoxic flavonolignans and flavones from Verbascum sinaiticum leaves. Phytochemistry, 1993, 34(3), 839-841.
[15]
Miyase, T.; Horikoshi, C.; Yabe, S.; Miyasaka, S.; Melek, F.R.; Kusano, G. Saikosaponin homologues from Verbascum spp. The structures of mulleinsaponins I-VII. Chem. Pharm. Bull., 1997, 45(12), 2029-2033.
[16]
Mahmoud, S.M.; Abdel-Azim, N.S.; Shahat, A.A.; Ismail, S.I.; Hammouda, F.M. Phytochemical and biological studies on Verbascum sinaiticum growing in Egypt. Nat. Prod. Sci., 2007, 13(3), 186.
[17]
Blois, M.S. Antioxidant determinations by the use of a stable free radical. Nature, 1958, 26, 1199-1200.
[18]
Brand-Williams, W.; Cuvelier, M.E.; Berset, C. Use of a free radical method to evaluate antioxidant activity. LWT -. Food Sci. Tech., 1995, 28, 25-30.
[19]
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 1999, 26, 1231-1237.
[20]
Thaipong, K.; Boonprakob, U.; Crosby, K. Comparison of ABTS, DPPH, FRAP and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal., 2006, 19, 669-675.
[21]
Oyaizu, M. Studies on product of browning reaction prepared from glucose amine. Japan J. Nutr., 1986, 44, 307-315.
[22]
Andary, C.; Wylde, R.; Laffite, C.; Privat, G.; Winternitz, F. Structures of verbascoside and orobanchoside, caffeic acid sugar esters from Orobanche rapum-genistae. Phytochemistry, 1982, 21(5), 1123-1127.
[23]
Sticher, O.; Salama, O.; Chaudhuri, R.K.; Winkler, T. Structural analysis of eukovoside, a new phenylpropanoid glycoside from Euphrasia rostkoviana Hayne. Helv. Chim. Acta, 1982, 65(5), 1538-1542.
[24]
Sasaki, H.; Nishimura, H.; Chin, M.; Mitsuhashi, H. Hydroxycinnamic acid esters of phenethylalcohol glycosides from Rehmannia glutinosa var. purpurea. Phytochemistry, 1989, 28(3), 875-879.
[25]
Imakura, Y.; Kobayashi, S.; Mima, A. Bitter phenyl propanoid glycosides from Campsis chinensis. Phytochemistry, 1985, 24(1), 139-146.
[26]
Froelich, S.; Gupta, M.P.; Siems, K.; Jenett-Siems, K. Phenylethanoid glycosides from Stachytarpheta cayennensis (Rich.) Vahl, Verbenaceae, a traditional antimalarial medicinal plant. Revista Brasil. Farmacogn., 2008, 18(4), 517-520.
[27]
Miyase, T.; Koizumi, A.; Ueno, A.; Noro, T.; Kuroyanagi, M.; Fukushima, S.; Takemoto, T. Studies on the acyl glycosides from Leucoseptrum japonicum (Miq.) Kitamura et Murata. Chem. Pharm. Bull., 1982, 30(8), 2732-2737.
[28]
Sasaki, H.; Nishimura, H.; Morota, T.; Chin, M.; Mitsuhashi, H.; Komatsu, Y.; Maruyama, H.; Guo-rui, T.; Wei, H.; Yu-lang, X. Immunosuppressive Principles of Rehmannia glutinosa var. hueichingensis. Planta Med., 1989, 55(5), 458-462.
[29]
Si, C.L.; Deng, X.J.; Liu, Z.; Kim, J.K.; Bae, Y.S. Studies on the phenylethanoid glycosides with anti-complement activity from Paulownia tomentosa var. tomentosa wood. J. Asian Nat. Prod. Res., 2008, 10(11), 1003-1008.
[30]
Wu, J.; Zhang, S.; Xiao, Q.; Li, Q.; Huang, J.; Long, L.; Huang, L. Phenylethanoid and aliphatic alcohol glycosides from Acanthus ilicifolius. Phytochemistry, 2003, 63(4), 491-495.
[31]
Fu, G.; Pang, H.; Wong, Y.H. Naturally occurring phenylethanoid glycosides: Potential leads for new therapeutics. Curr. Med. Chem., 2008, 15(25), 2592-2613.
[32]
Kitagawa, S.; Tsukamoto, H.; Hisada, S.; Nishibe, S. Studies on the Chinese Crude Drug “Forsythiae Fructus” VII. A New Caffeoyl Glycoside from Forsythia viridissima. Chem. Pharm. Bull., 1984, 32(3), 1209-1213.
[33]
Alipieva, K.; Korkina, L.; Orhan, I.E.; Georgiev, M.I. Verbascoside-A review of its occurrence, (bio) synthesis and pharmacological significance. Biotechnol. Adv., 2014, 32(6), 1065-1076.
[34]
Jimenez, C.; Riguera, R. Phenylethanoid glycosides in plants: structure and biological activity. Nat. Prod. Rep., 1994, 11(6), 591-606.

Rights & Permissions Print Cite
Article Metrics
15
© 2024 Bentham Science Publishers | Privacy Policy