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Current Analytical Chemistry

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ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

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Research Article

Melamine Detection in Food matrices employing Chicken Antibody (IgY): A Comparison between Colorimetric and Chemiluminescent Methods

Author(s): Umesh Sushma, Alok K. Srivastava and Manonmani H. Krishnan*

Volume 15, Issue 6, 2019

Page: [668 - 677] Pages: 10

DOI: 10.2174/1573411015666181205120323

Price: $65

Abstract

Background: Melamine, contains 67% nitrogen by mass, and is adulterated in foods to uplift false protein. There is an urgent requirement to develop fast screening techniques for monitoring melamine in foods.

Objectives: To develop rapid, high throughput detection techniques for melamine in the food matrix.

Methods: IgY antibodies were developed against melamine in the hen, isolated and used for detection of melamine. The detection by colorimetric and chemiluminescent methods was compared.

Results: The detection range for melamine was 1 ng-25 µg by the colorimetric method and 10 fg/mL-25 ng/mL by the chemiluminescent method. There was a very low matrix effect, where the recovery was 86 to 106 % by colorimetric method and 71 to 98 % by the chemiluminescent method.

Conclusion: Both colorimetric and chemiluminescent methods could be employed for the fast and consistent melamine detection in the food matrix.

Keywords: Chemiluminescence, colorimetric, ELISA, enzyme-linked immunosorbent assay (ELISA), IgY antibodies, melamine.

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