Login Register Cart 0
Generic placeholder image

Current Analytical Chemistry

Editor-in-Chief

ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

A New Multiple-Dilution-Assays Method for Determining Glucose Yield from Enzymatic Saccharification of Biomass at High-Solids Loadings

Author(s): Han Zhang, Hao Liu*, Jianliang Sun, Mingqian Mai, Shiyu Fu and Xiangyang Xu

Volume 15, Issue 6, 2019

Page: [685 - 693] Pages: 9

DOI: 10.2174/1573411014666180518085855

Price: $65

Abstract

Background: Determination of the accurate mass of glucose generated from high-solids biomass saccharification is vital but problematic due to the uncertainty of liquid volume and slurry density.

Methods: Herein, a new multiple-dilution-assays method was established to deduce the accurate glucose mass from the hydrolyzing biomass slurry.

Results: This method was applicable for slurries of pretreated corn stover with a solids consistency up to 30 wt%, showing a high accuracy and good reproducibility. Dryness did not interfere with the accuracy. Ethanol at a high level, e.g. 10%, caused only a small negative error (<2%). This method can be used in either single- or fed-batch high-solids biomass saccharification, allowing to quantify the maldistribution of glucose in the slurry.

Conclusion: The significant advantage of the present method was that only one single variable, glucose concentration, was to be determined, rendering it unnecessary to wash the insoluble or to measure the changing liquid density.

Keywords: Multiple dilution assays (MDA), glucose yield, high-solids, enzymatic hydrolysis (saccharification), biomass, ethanol.

« Previous Next »
Graphical Abstract

[1]
Daystar, J.; Reeb, C.; Gonzalez, R.; Venditti, R.; Kelley, S.S. Environmental life cycle impacts of cellulosic ethanol in the Southern US produced from loblolly pine, eucalyptus, unmanaged hardwoods, forest residues, and switchgrass using a thermochemical conversion pathway. Fuel Process. Technol., 2015, 138, 164-174.
[2]
Gombert, A.K.; van Maris, A.J. Improving conversion yield of fermentable sugars into fuel ethanol in 1st generation yeast-based production processes. Curr. Opin. Biotechnol., 2015, 33, 81-86.
[3]
Ho, D.P.; Ngo, H.H.; Guo, W. A mini review on renewable sources for biofuel. Bioresour. Technol., 2014, 169, 742-749.
[4]
Maity, S.K. Opportunities, recent trends and challenges of integrated biorefinery: part I. Renew. Sustainable. Energy Rev., 2015, 43, 1427-1445.
[5]
Mood, S.H.; Golfeshan, A.H.; Tabatabaei, M.; Jouzani, G.S.; Najafi, G.H.; Gholami, M. Lignocellulosic biomass to bioethanol, a comprehensive review with a focus on pretreatment. Renew. Sustainable. Energy Rev., 2013, 27, 77-93.
[6]
Narra, M.; Balasubramanian, V.; James, J.P. Enhanced enzymatic hydrolysis of mild alkali pre-treated rice straw at high-solid loadings using in-house cellulases in a bench scale system. Bioproc Biosyst. Eng., 2016, 39(6), 993-1003.
[7]
Modenbach, A.A.; Nokes, S.E. The use of high‐solids loadings in biomass pretreatment-a review. Biotechnol. Bioeng., 2012, 109(6), 1430-1442.
[8]
Kremer, F.; Blank, L.M.; Jones, P.R.; Akhtar, M.K. A comparison of the microbial production and combustion characteristics of three alcohol biofuels: Ethanol, 1-butanol, and 1-octanol. Front. Bioeng. Biotechnol., 2015, 3, 112.
[9]
Chen, X.; Kuhn, E.; Jennings, E.W.; Nelson, R.; Tao, L.; Zhang, M. DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g· L− 1) during enzymatic hydrolysis and high ethanol concentrations (> 10% v/v) during fermentation without hydrolysate purification or concentration. Energy Environ. Sci., 2016, 9, 1237-1245.
[10]
López-Linares, J.C.; Romero, I.; Cara, C.; Ruiz, E.; Moya, M.; Castro, E. Bioethanol production from rapeseed straw at high solids loading with different process configurations. Fuel, 2014, 122, 112-8.
[11]
Kristensen, J.B.; Felby, C.; Jørgensen, H. Determining yields in high solids enzymatic hydrolysis of biomass. Appl. Biochem. Biotechnol., 2009, 156, 127-132.
[12]
Nguyen, T.Y.; Cai, C.M.; Osman, O.; Kumar, R.; Wyman, C.E. CELF pretreatment of corn stover boosts ethanol titers and yields from high solids SSF with low enzyme loadings. Green Chem., 2016, 18, 1581-1589.
[13]
Hodge, D.B.; Karim, M.N.; Schell, D.J.; McMillan, J.D. Model based fed-batch for high-solids enzymatic cellulose hydrolysis. Appl. Biochem. Biotechnol., 2009, 152, 88-107.
[14]
Olsen, S.N.; Lumby, E.; McFarland, K.; Borch, K.; Westh, P. Kinetics of enzymatic high-solid hydrolysis of lignocellulosic biomass studied by calorimetry. Appl. Biochem. Biotechnol., 2011, 163, 626-635.
[15]
Zhu, Y.; Malten, M.; Torry-Smith, M.; McMillan, J.D.; Stickel, J.J. Calculating sugar yields in high solids hydrolysis of biomass. Bioresour. Technol., 2011, 102, 2897-2903.
[16]
Roche, C.M.; Dibble, C.J.; Stickel, J.J. Laboratory-scale method for enzymatic saccharification of lignocellulosic biomass at high solids loadings. Biotechnol. Biofuels, 2009, 2, 1.
[17]
Roche, C.M.; Dibble, C.J.; Knutsen, J.S.; Stickel, J.J.; Liberatore, M.W. Particle concentration and yield stress of biomass slurries during enzymatic hydrolysis at high-solids loadings. Biotechnol. Bioeng., 2009, 104, 290-300.
[18]
Ghose, T. Measurement of cellulase activities. Pure Appl. Chem., 1987, 59, 257-268.
[19]
Palmqvist, B.; Lidén, G. Torque measurements reveal large process differences between materials during high solid enzymatic hydrolysis of pretreated lignocellulose. Biotechnol. Biofuels, 2012, 5, 1.
[20]
Luo, X.; Zhu, J. Effects of drying-induced fiber hornification on enzymatic saccharification of lignocelluloses. Enzyme Microb. Technol., 2011, 48, 92-99.
[21]
Vani, S.; Sukumaran, R.K.; Savithri, S. Prediction of sugar yields during hydrolysis of lignocellulosic biomass using artificial neural network modeling. Bioresour. Technol., 2015, 188, 128-135.
[22]
Eckelt, J.; Richardt, D.; Schuster, K.C.; Wolf, B.A. Thermodynamic interactions of natural and of man-made cellulose fibers with water. Cellulose, 2010, 17, 1079-93.
[23]
Przybysz, P.; Dubowik, M.; Kucner, M.A.; Przybysz, K.; Buzała, K.P. Contribution of hydrogen bonds to paper strength properties. PloS One, 2016, 11e0155809

Rights & Permissions Print Cite
Article Metrics
22
2
1
1
© 2024 Bentham Science Publishers | Privacy Policy