Abstract
Background: Curcumin is a potent anticancer agent and has great potential efficacy against different types of cancers. A major disadvantage of curcumin, however, is its poor solubility and bioavailability.
Objective: The aim of the present work is to synthesize chitosan and curcumin-loaded chitosan nanoparticles and their characterization through various physicochemical methods and cellular uptake in cervical cancer cell line SiHa.
Method: Chitosan nanoparticles were synthesized through the method of ionic gelation of chitosan with sodium Tripolyphosphate (TPP). In addition, the internal structure of chitosan nanoparticles and curcumin loaded chitosan nanoparticles were characterized by DLS, UV-Visible spectrophotometer, DSC, LCMS and LDH assay.
Results: The studies presented demonstrate that curcumin-loaded chitosan nanoparticles showed increased uptake in the SiHa cells as compared to free curcumin and chitosan nanoparticles did not show any significant uptake in SiHa cell line. The curcumin-loaded chitosan nanoparticles released more lactate and lower ATP as compared to native curcumin in cervical cancer lines such as SiHa, CaSki and HeLa.
Conclusion: Thus, chitosan based curcumin nanoparticles could be used as a potent vector / delivery agent for drug targeting in the treatment of cervical cancer.
Keywords: Curcumin, chitosan nanoparticles, cervical cancer, cellular uptake, LDH assay, SiHa cells.
Anti-Cancer Agents in Medicinal Chemistry
Title:Physicochemical Characterization of Curcumin Loaded Chitosan Nanoparticles: Implications in Cervical Cancer
Volume: 18 Issue: 8
Author(s): Md. Asad Khan*, Md. Zafaryab, Syed H. Mehdi, Irfan Ahmad and Md. Moshahid A. Rizvi*
Affiliation:
- Department of Biochemistry, Faculty of Dentistry, Jamia Millia Islamia, New Delhi,India
- Department of Biosciences, Jamia Millia Islamia, New Delhi,India
Keywords: Curcumin, chitosan nanoparticles, cervical cancer, cellular uptake, LDH assay, SiHa cells.
Abstract: Background: Curcumin is a potent anticancer agent and has great potential efficacy against different types of cancers. A major disadvantage of curcumin, however, is its poor solubility and bioavailability.
Objective: The aim of the present work is to synthesize chitosan and curcumin-loaded chitosan nanoparticles and their characterization through various physicochemical methods and cellular uptake in cervical cancer cell line SiHa.
Method: Chitosan nanoparticles were synthesized through the method of ionic gelation of chitosan with sodium Tripolyphosphate (TPP). In addition, the internal structure of chitosan nanoparticles and curcumin loaded chitosan nanoparticles were characterized by DLS, UV-Visible spectrophotometer, DSC, LCMS and LDH assay.
Results: The studies presented demonstrate that curcumin-loaded chitosan nanoparticles showed increased uptake in the SiHa cells as compared to free curcumin and chitosan nanoparticles did not show any significant uptake in SiHa cell line. The curcumin-loaded chitosan nanoparticles released more lactate and lower ATP as compared to native curcumin in cervical cancer lines such as SiHa, CaSki and HeLa.
Conclusion: Thus, chitosan based curcumin nanoparticles could be used as a potent vector / delivery agent for drug targeting in the treatment of cervical cancer.
Export Options
About this article
Cite this article as:
Khan Asad Md.*, Zafaryab Md., Mehdi H. Syed , Ahmad Irfan and Rizvi Moshahid A. Md. *, Physicochemical Characterization of Curcumin Loaded Chitosan Nanoparticles: Implications in Cervical Cancer, Anti-Cancer Agents in Medicinal Chemistry 2018; 18 (8) . https://dx.doi.org/10.2174/1871520618666180412114352
DOI https://dx.doi.org/10.2174/1871520618666180412114352 |
Print ISSN 1871-5206 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5992 |
Call for Papers in Thematic Issues
Induction of cell death in cancer cells by modulating telomerase activity using small molecule drugs
Telomeres are distinctive but short stretches present at the corners of chromosomes and aid in stabilizing chromosomal makeup. Resynthesis of telomeres supported by the activity of reverse transcriptase ribonucleoprotein complex telomerase. There is no any telomerase activity in human somatic cells, but the stem cells and germ cells undergone telomerase ...read more
Role of natural compounds as anti anti-cancer agents
Cancer is considered the leading cause of worldwide mortality, accounting for nearly 10 million deaths in 2022. Cancer outcome can be improved through an appropriate screening and early detection and through an efficient clinical treatment. Chemotherapy remains an important approach in treatment o f several types of cancers, even though ...read more
Signaling and enzymatic modulators in cancer treatment
Cancer accounts for nearly 10 million deaths in 2022 and is considered the leading cause of worldwide mortality. Cancer outcome can be improved through an appropriate screening and early detection and through an efficient clinical treatment. Chemotherapy, radiotherapy and surgery are the most important approach for the treatment of several ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
MiRNAs in Human Cancers: The Diagnostic and Therapeutic Implications
Current Pharmaceutical Design Polysulfated/Sulfonated Compounds for the Development of Drugs at the Crossroad of Viral Infection and Oncogenesis
Current Pharmaceutical Design Pin1: A New Genetic Link between Alzheimer’s Disease, Cancer and Aging
Current Aging Science The Human Desmin Promoter Drives Robust Gene Expression for Skeletal Muscle Stem Cell-Mediated Gene Therapy
Current Gene Therapy Significance of P2X7 Receptor Variants to Human Health and Disease
Recent Patents on DNA & Gene Sequences Vitamin D Receptor is a Novel Drug Target for Ovarian Cancer Treatment
Current Cancer Drug Targets Nicotine Vaccines
CNS & Neurological Disorders - Drug Targets Review on Documented Medicinal Plants used for the Treatment of Cancer
Current Traditional Medicine Glioma: Tryptophan Catabolite and Melatoninergic Pathways Link microRNA, 14-3- 3, Chromosome 4q35, Epigenetic Processes and other Glioma Biochemical Changes
Current Pharmaceutical Design Synthesis and Anti-tumor Activity Evaluation of Gallic Acid-mangiferin Hybrid Molecule
Medicinal Chemistry Patents on Therapeutic and Cosmetic Applications of Bioactives of Crocus Sativus L. and their Production through Synthetic Biology Methods: A Review
Recent Patents on Biotechnology Cultured Adult Animal Neurons and Schwann Cells Give Us New Insights into Diabetic Neuropathy
Current Diabetes Reviews Effect of Monoamine Oxidase Inhibition on Rewarding Effects of Nicotine in Rodents
Current Drug Abuse Reviews Three Amino Acid Derivatives of Valproic Acid: Design, Synthesis, Theoretical and Experimental Evaluation as Anticancer Agents
Anti-Cancer Agents in Medicinal Chemistry MicroRNAs Patents: The Road From Bench to Bedsides for Cancer Treatment
Recent Patents on DNA & Gene Sequences Targeted Vascular Drug Delivery in Cerebral Cancer
Current Pharmaceutical Design Synthesis, Characterization, and Inducing Tumor Cell Apoptosis of Two Ru(II) Complexes Containing Guanidinium as Ligands
Anti-Cancer Agents in Medicinal Chemistry Approaches to Improve Efficiency of Dendritic Cell-based Therapy of High Grade Gliomas
Current Pharmaceutical Design Crotamine, a Small Basic Polypeptide Myotoxin from Rattlesnake Venom with Cell-Penetrating Properties
Current Pharmaceutical Design Manipulation of Dendritic Cells for Tumor Immunity
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry