Abstract
Background: Low back pain is a common problem, mainly caused by intervertebral disc degeneration (IDD). An important pathophysiological characteristic of IDD is the loss of homeostatic balance of the extracellular matrix metabolism. Interleukin-1β (IL-1β) is one of the inflammatory mediators stimulating the degradation of extracellular matrix in the nucleus pulposus (NP) and contributing to IDD pathogenesis. Icariin, which is isolated from Epimedium brevicornum, acts as an anti-inflammatory drug.
Objective: This study aimed to explore the pharmacological effects of icariin in IDD by simulating NP inflammation in vitro.
Method: Human NP cells were isolated and cultured in vitro. NP cells were pretreated with icariin (0.1, 1 and 10 µM) and stimulated by IL-1β (10 ng/ml). The concentration of Prostaglandin E2 was determined by enzymelinked immunosorbent assay. Nitric oxide was determined by Griess reagent assay. The expression of cyclooxygenase- 2 (COX-2), inducible nitric oxide synthase (iNOS), degrading enzymes, collagen II, aggrecan, mitogenactivated protein kinase (MAPK), and nuclear factor-kappa B (NF-κB)-related signaling molecules was assessed via western blotting.
Results: IL-1β induced pronounced expression of COX-2 and iNOS, and stimulated production of prostaglandin E2 and nitric oxide. Icariin exhibited significant anti-inflammatory effect, inhibiting IL-1β-induced production of degrading enzymes, as well as extracellular matrix reduction. Finally, icariin suppressed IL-1β-induced activation of MAPK- and NF-κB-related signaling pathways.
Conclusion: The present findings suggest that icariin may have a protective effect on NP cells. The antiinflammatory effect may contribute to the therapeutic action of icariin in IDD.
Keywords: Icariin, intervertebral disc degeneration, extracellular matrix, mitogen-activated protein kinase, MAPK, nuclear factor-kappa B, NF-kB.
Current Pharmaceutical Design
Title:Icariin Attenuates Interleukin-1β-Induced Inflammatory Response in Human Nucleus Pulposus Cells
Volume: 23 Issue: 39
Author(s): Wenbin Hua, Yukun Zhang, Xinghuo Wu, Liang Kang, Ji Tu, Kangcheng Zhao, Shuai Li, Kun Wang, Yu Song, Rongjin Luo, Zengwu Shao, Shuhua Yang and Cao Yang*
Affiliation:
- Department of Orthopaedics, Union Hospial, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China
Keywords: Icariin, intervertebral disc degeneration, extracellular matrix, mitogen-activated protein kinase, MAPK, nuclear factor-kappa B, NF-kB.
Abstract: Background: Low back pain is a common problem, mainly caused by intervertebral disc degeneration (IDD). An important pathophysiological characteristic of IDD is the loss of homeostatic balance of the extracellular matrix metabolism. Interleukin-1β (IL-1β) is one of the inflammatory mediators stimulating the degradation of extracellular matrix in the nucleus pulposus (NP) and contributing to IDD pathogenesis. Icariin, which is isolated from Epimedium brevicornum, acts as an anti-inflammatory drug.
Objective: This study aimed to explore the pharmacological effects of icariin in IDD by simulating NP inflammation in vitro.
Method: Human NP cells were isolated and cultured in vitro. NP cells were pretreated with icariin (0.1, 1 and 10 µM) and stimulated by IL-1β (10 ng/ml). The concentration of Prostaglandin E2 was determined by enzymelinked immunosorbent assay. Nitric oxide was determined by Griess reagent assay. The expression of cyclooxygenase- 2 (COX-2), inducible nitric oxide synthase (iNOS), degrading enzymes, collagen II, aggrecan, mitogenactivated protein kinase (MAPK), and nuclear factor-kappa B (NF-κB)-related signaling molecules was assessed via western blotting.
Results: IL-1β induced pronounced expression of COX-2 and iNOS, and stimulated production of prostaglandin E2 and nitric oxide. Icariin exhibited significant anti-inflammatory effect, inhibiting IL-1β-induced production of degrading enzymes, as well as extracellular matrix reduction. Finally, icariin suppressed IL-1β-induced activation of MAPK- and NF-κB-related signaling pathways.
Conclusion: The present findings suggest that icariin may have a protective effect on NP cells. The antiinflammatory effect may contribute to the therapeutic action of icariin in IDD.
Export Options
About this article
Cite this article as:
Hua Wenbin , Zhang Yukun , Wu Xinghuo, Kang Liang , Tu Ji, Zhao Kangcheng, Li Shuai , Wang Kun , Song Yu , Luo Rongjin, Shao Zengwu , Yang Shuhua and Yang Cao *, Icariin Attenuates Interleukin-1β-Induced Inflammatory Response in Human Nucleus Pulposus Cells, Current Pharmaceutical Design 2017; 23 (39) . https://dx.doi.org/10.2174/1381612823666170615112158
DOI https://dx.doi.org/10.2174/1381612823666170615112158 |
Print ISSN 1381-6128 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4286 |
Call for Papers in Thematic Issues
"Tuberculosis Prevention, Diagnosis and Drug Discovery"
The Nobel Prize-winning discoveries of Mycobacterium tuberculosis and streptomycin have enabled an appropriate diagnosis and an effective treatment of tuberculosis (TB). Since then, many newer diagnosis methods and drugs have been saving millions of lives. Despite advances in the past, TB is still a leading cause of infectious disease mortality ...read more
Current Pharmaceutical challenges in the treatment and diagnosis of neurological dysfunctions
Neurological dysfunctions (MND, ALS, MS, PD, AD, HD, ALS, Autism, OCD etc..) present significant challenges in both diagnosis and treatment, often necessitating innovative approaches and therapeutic interventions. This thematic issue aims to explore the current pharmaceutical landscape surrounding neurological disorders, shedding light on the challenges faced by researchers, clinicians, and ...read more
Emerging and re-emerging diseases
Faced with a possible endemic situation of COVID-19, the world has experienced two important phenomena, the emergence of new infectious diseases and/or the resurgence of previously eradicated infectious diseases. Furthermore, the geographic distribution of such diseases has also undergone changes. This context, in turn, may have a strong relationship with ...read more
Melanoma and Non-Melanoma Skin Cancer Treatment: Standard of Care and Recent Advances
In this thematic issue, we aim to provide a standard of care of the diagnosis and treatment of melanoma and non-melanoma skin cancer. The editor will invite authors from different countries who will write review articles of melanoma and non-melanoma skin cancers. The Diagnosis, Staging, Surgical Treatment, Non-Surgical Treatment all ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
New Insights into the Treatment of Multiple Myeloma with Histone Deacetylase Inhibitors
Current Pharmaceutical Design Protein Transduction Domains and their Utility in Gene Therapy
Current Gene Therapy pH-Dependent Behavior of Novel Gellan Beads Loaded with Naproxen
Current Drug Delivery Antisense Technology: A Selective Tool for Gene Expression Regulation and Gene Targeting
Current Pharmaceutical Biotechnology Potential Mechanisms for Increased HIV-1 Transmission Across the Endocervical Epithelium During C. trachomatis Infection
Current HIV Research The Effects of D-aspartate on Neurosteroids, Neurosteroid Receptors, and Inflammatory Mediators in Experimental Autoimmune Encephalomyelitis
Endocrine, Metabolic & Immune Disorders - Drug Targets Evaluation of Glycated Haemoglobin (HbA1c) Level in Type 2 Diabetic Chronic HCV Non-cirrhotic Treatment-Naïve Egyptian Patients Eradicated with Sofosbuvir Plus Daclatasvir
Current Diabetes Reviews Cellular and Physiological Effects of Soy Flavonoids
Mini-Reviews in Medicinal Chemistry Evaluation of Extemporaneously Manufactured Topical Gels Containing Aceclofenac on Inflammation and Hyperalgesia in Rats
Current Drug Delivery Molecular Aspects of Intestinal Radiation-Induced Fibrosis
Current Molecular Medicine CXCL3 is a Potential Target for Breast Cancer Metastasis
Current Cancer Drug Targets Aspirin: from a Historical Perspective
Recent Patents on Cardiovascular Drug Discovery Genomic and Pharmacogenomic Biomarkers of Parkinson’s Disease
Current Drug Metabolism Prognostic and Predictive Molecular Markers in Cutaneous Malignant Melanoma: The First Step Toward Personalized Medicine
Current Pharmacogenomics and Personalized Medicine In Vitro and In Vivo Sirolimus for Modulation of Allogeneic Hematopoietic Stem Cell Transplantation
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents N-Glycosylation of Plant-produced Recombinant Proteins
Current Pharmaceutical Design Cognitive Identity in Schizophrenia: Vision, Space, and Body Perception from Prodrome to Syndrome
Current Psychiatry Reviews Xanthomicrol: A Comprehensive Review of Its Chemistry, Distribution, Biosynthesis and Pharmacological Activity
Mini-Reviews in Medicinal Chemistry Modulation of the Immune Response by the Cholera-like Enterotoxins
Current Topics in Medicinal Chemistry CC-Chemokine Receptors in Chronic Obstructive Pulmonary Disease
Inflammation & Allergy - Drug Targets (Discontinued)