Low Concentrations of Cationic PAMAM Dendrimers Affect Lymphocyte Respiration in In vitro Studies

Title:Low Concentrations of Cationic PAMAM Dendrimers Affect Lymphocyte Respiration in In vitro Studies

Volume: 18 Issue: 2

Author(s): Magdalena Labieniec-Watala, Marzena Szwed, Joanna Hertel and Ewelina Wisnik

Affiliation:

Keywords: Biomaterials, mitochondria, morphology, nanotechnology, PAMAM dendrimers, toxicity.

Abstract: Background: In this study, the effect of low concentrations of poly(amido)amine dendrimers (G2-G4) on human lymphocytes was studied. Some works revealed that PAMAMs can adversely affect the morphology of blood components and mitochondria functions. In this context, the present report aimed to investigate the in vitro cationic dendrimers’ effect on mitochondrial respiration and cell morphology in lymphocytes isolated from human blood.

Methods: To monitor the mitochondrial changes, the high-resolution respirometer was used, whereas the cell morphology was analyzed using a flow cytometer and fluorescence microscopy.

Results: The concentration-dependent dendrimers’ influence on lymphocytes morphology was shown. Changes in mitochondrial respiration revealed the concentration- and generation-dependent differences between dendrimer activity. There were no alterations in the routine respiration and in the state of the inner mitochondrial membrane (L/E), but decreased ADP- and FCCP-stimulated respirations were detected after treatment with G3 and G4 dendrimers. The markers of mitochondrial membrane integrity (RCR) and OXPHOS efficiency (P/E) significantly decreased regardless of the dendrimer generation used.

Conclusion: Based on these in vitro evaluations, we state that cationic PAMAM dendrimers can impair both the morphology and the bioenergetics of human lymphocytes, even when used at low concentrations and in a short time (up to 1 h). However, these results do not imply that similar findings could be possible for in vivo observations.

Cite this article as:

Labieniec-Watala Magdalena, Szwed Marzena, Hertel Joanna and Wisnik Ewelina, Low Concentrations of Cationic PAMAM Dendrimers Affect Lymphocyte Respiration in In vitro Studies, Current Pharmaceutical Biotechnology 2017; 18 (2) . https://dx.doi.org/10.2174/1389201017666161229153018

DOI https://dx.doi.org/10.2174/1389201017666161229153018	Print ISSN 1389-2010
Publisher Name Bentham Science Publisher	Online ISSN 1873-4316