Background: Renal dysfunction is a common complication in type 2 diabetes mellitus patients
associated with oxidative damage which could be characterized by 8-iso-prostaglandin F2α and
hydrogen peroxide level as oxidative stress markers.
Objective: The aim of our study is to determine if there is a difference in 8-iso-prostaglandin F2α and
hydrogen peroxide levels between sulfonylurea and combination of metformin-sulfonylurea in diabetic
patients. We also wanted to determine if these oxidative stress markers correlate with the estimated
Glomerular Filtration Rate (eGFR).
Subjects and Methods: We conducted a cross-sectional study with inclusion of 55 patients with type 2
diabetes mellitus in Dr. Sitanala Tangerang Hospital, Indonesia with purposive sampling. The value of
eGFR was obtained by serum creatinine levels, while the level of 8-iso-prostaglandin F2α was measured
by ELISA and urinary hydrogen peroxide using FOX-1 (Ferrous Ion Oxidation Xylenol Orange 1).
Results: There was no difference in 8-iso-prostaglandin F2α and hydrogen peroxide level between the
two groups (p=0.088 and p=0.848). Moreover, there was no difference in eGFR values between the two
groups, measured by Cockroft-Gault, MDRD, and CKD-EPI. 8-iso-prostaglandin F2α (n=55) was positively
correlated with eGFR based on Cockroft-Gault (r=0.382; p=0.009), whereas urinary hydrogen
peroxide (n=47) also generate significant positive correlation with eGFR based on the MDRD equation
(r=0.326; p=0.021). Linear regression analysis showed that 8-iso-prostaglandin F2α is the most
predictive factor and the only significant factor for eGFR in Cockroft-Gault, MDRD and also CKDEPI,
even after controlled by gender, age, BMI, HbA1c, systole, and H2O2.
Conclusion: The two treatments did not have any significant differences in antioxidant activity. However,
an increase of urinary 8-iso-prostaglandin F2 and hydrogen peroxide which correlates with
eGFR in the total sample may play a significant role in the pathophysiology of diabetic nephropathy.