Non-Clinical Screening of Cyto-Genotoxicologic Status of Praziquantel Nanoemulsion

Title:Non-Clinical Screening of Cyto-Genotoxicologic Status of Praziquantel Nanoemulsion

Volume: 14 Issue: 1

Author(s): Muhammad T. Islam*, Leticia Streck, Samara W.C. Silva, Ana M.O.F. da Mata, Marcus V.O.B. de Alencar, Ricardo M. de Carvalho, Arnobio A. da Silva-Junior and Ana A.C. Melo-Cavalcante

Affiliation:

• Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, 700000,Vietnam

Keywords: Cytotoxicity, mutagenicity, praziquantel nanoemulsion, enzymes, hypotension, budding parenchyma.

Abstract: Background: Prazequantel (Prz) is an important medication in humans and animals. It acts against a number of pathogens. In this study, Prz nanoemulsion (PrzNE) was prepared and evaluated for toxic, cytotoxic and mutagenic potentials.

Materials and Methods: The cytotoxic effect of PrzNE was observed in Artemia salina and Allium cepa, while toxicity and mutegenicity in A. cepa test system. Potassium di-chromate (K2Cr2O7) and copper sulphate (CuSO4.5H2O) were taken as standards for A. salina and A. cepa test, respectively.

Results: PrzNE at 16 µM caused 100% mortality of the brine shripms. It exerted a concentrationdependent toxic and cytotoxic effects in A. cepa and A. salina. In comparison to the CuSO4.5H2O, PrzNE produced less chromosomal aberrations (CA), where chromosomal fragments/loose and bridges were most frequently observed.

Conclusion: PrzNE may readily cross the cell membranes of A. cepa and A. salina, increase the permeability of the cell membrane and/or exert an oxidative stress, thus causes a cytotoxic effect. The PrzNE was more cytotoxic than mutagenic in nature.

Cite this article as:

Islam T. Muhammad *, Streck Leticia, Silva W.C. Samara, da Mata M.O.F. Ana, de Alencar V.O.B. Marcus, de Carvalho M. Ricardo, da Silva-Junior A. Arnobio and Melo-Cavalcante A.C. Ana, Non-Clinical Screening of Cyto-Genotoxicologic Status of Praziquantel Nanoemulsion, Current Bioactive Compounds 2018; 14 (1) . https://dx.doi.org/10.2174/1573407213666161121162753