An Improved and Validated HPLC Method for the Determination of Methylprednisolone Sodium Succinate and its Degradation Products in Nanoparticles

Title:An Improved and Validated HPLC Method for the Determination of Methylprednisolone Sodium Succinate and its Degradation Products in Nanoparticles

Volume: 13 Issue: 2

Author(s): Yasemin Karabey-Akyurek, Emirhan Nemutlu, Erem Bilensoy and Levent Oner

Affiliation:

Keywords: Methylprednisolone sodium succinate, HPLC, validation, nanoparticles, pharmaceuticals.

Abstract: Background: A reversed-phase high-performance liquid chromatographic method is developed for quantitative analysis of methylprednisolone sodium succinate (MPSS) in nanoparticles.

Method: An ACE 5AQ column (4.6 × 210 mm, 5 µm, Advanced Chromatography Technologies) was used as stationary phase and phosphate buffered saline (PBS, pH 7.4): acetonitrile (70:30 v/v) at a flowrate of 1.5 mL min^-1 was used as mobile phase. The retention time of MPSS was around 5 min. The UV detection was performed at 243 nm. The proposed method was precise, accurate, and linear over the concentration range of analysis (1-100 µg mL^-1, R²=0.9999) with a limit of detection and a limit of quantification of 0.03 and 0.12 µg mL^-1, respectively.

Result: No interference was observed from the nanoparticle formulation ingredients. The method validated was applied for the analysis of MPSS in-vitro release samples of polycaprolactone (PCL) nanoparticles.

Cite this article as:

Karabey-Akyurek Yasemin, Nemutlu Emirhan, Bilensoy Erem and Oner Levent, An Improved and Validated HPLC Method for the Determination of Methylprednisolone Sodium Succinate and its Degradation Products in Nanoparticles, Current Pharmaceutical Analysis 2017; 13 (2) . https://dx.doi.org/10.2174/1573412913666161107155008

DOI https://dx.doi.org/10.2174/1573412913666161107155008	Print ISSN 1573-4129
Publisher Name Bentham Science Publisher	Online ISSN 1875-676X