Development and Validation of a Rapid UV-HPLC Method for the Determination of Anidulafungin in Perfusion Solution

Title:Development and Validation of a Rapid UV-HPLC Method for the Determination of Anidulafungin in Perfusion Solution

Volume: 13 Issue: 3

Author(s): Gabriel Estan-Cerezo*, Francisco Jose Rodriguez-Lucena*, Ana Cristina Murcia-López, Carmen Matoses Chirivella, Vanesa Escudero-Ortiz, Arantxa Andujar-Mateos and Andres Navarro-Ruiz

Affiliation:

• Servicio de Farmacia-Hospital General Universitario de Elche-Fisabio, Elche, Alicante,Spain

• Servicio de Farmacia-Hospital General Universitario de Elche-Fisabio, Elche, Alicante,Spain

Keywords: Anidulafungin, antifungal agents, echinocandins, HPLC, validation studies.

Abstract: Background: Anidulafungin is an antifungal agent. The development of determination techniques can be a useful tool to realize stability and quality control studies.

Methods: The determination was performed on an analytical Mediterranea SEA18 (15x0.4 cm, 5 μm) C18 column at 35 ºC. The selected wavelength was 304 nm. The mobile phase was a mixture of 0.037 M sodium dihydrogen phosphate buffer, acetonitrile and methanol (40:50:10, v/v/v) at a flow rate of 2.0 mL min–1. Dasatinib (12.5 μg mL-1) was used as internal standard.

Results: The assay enables the measurement of anidulafungin with a linear calibration curve (r2= 0.999) over the concentration range 15–300 μg mL-1. Accuracy, intra-day repeatability (n = 5), and inter-day precision (n = 3) were found to be satisfactory, being the accuracy 5.8% and precisions were intra-day and inter-day, 0.6% and 4.2%, respectively.

Conclusions: A rapid, simple and sensitive high-performance liquid chromatography (HPLC) method with ultra violet detection has been developed for quantification of anidulafungin in perfusion solution. The retention time was clearly minor than the previous published HPLC determination methods of anidulafungin.

Cite this article as:

Estan-Cerezo Gabriel*, Rodriguez-Lucena Jose Francisco*, Murcia-López Cristina Ana, Chirivella Matoses Carmen, Escudero-Ortiz Vanesa, Andujar-Mateos Arantxa and Navarro-Ruiz Andres, Development and Validation of a Rapid UV-HPLC Method for the Determination of Anidulafungin in Perfusion Solution, Current Pharmaceutical Analysis 2017; 13 (3) . https://dx.doi.org/10.2174/1573412912666160929170016

DOI https://dx.doi.org/10.2174/1573412912666160929170016	Print ISSN 1573-4129
Publisher Name Bentham Science Publisher	Online ISSN 1875-676X