Antioxidant Activities of N-ferrocenylmethyl-2- and -3-nitroaniline and Determination of their Binding Parameters with Superoxide Anion Radicals

Title:Antioxidant Activities of N-ferrocenylmethyl-2- and -3-nitroaniline and Determination of their Binding Parameters with Superoxide Anion Radicals

Volume: 13 Issue: 2

Author(s): Touhami Lanez and Hadia Hemmami

Affiliation:

Keywords: Ferrocene derivatives, antioxidant activities, cyclic voltammetry, superoxide anion radical, DPPH, binding parameters.

Abstract: Introduction: The antioxidant activity of two potential antioxidants N-ferrocenylmethyl-2- nitroaniline (2FMNA) and N-ferrocenylmethyl-3-nitroaniline (3FMNA) was evaluated using spectrophotometrical and electrochemical technics.

Methods: Spectrophotometrical assays were carried out using stable 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) and electrochemical assays were based on measuring the oxidation peak current of relatively stable superoxide anion radical O₂˙¯ electrochemically generated by reduction of commercial molecular oxygen in DMF.

Results: 2FMNA shows highest antioxidant activity in both methods DPPH and O₂˙¯ radicals scavenging activities (4.27 ± 0.01mM) and (9.38± 0.09 mM) respectively which is almost equal to that of standard antioxidant α-tocopherol (4.81± 0.03 mM) and (9.50± 0.09 mM). The diffusion coefficients of the free and O₂˙¯ bound forms of 2FMNA and 3FMNA were evaluated from Randles–Sevcik equation. Cyclic voltammetry based assay was developed for the calculation of binding parameters like binding constant, ratio of binding constants and binding free energy.

Cite this article as:

Lanez Touhami and Hemmami Hadia, Antioxidant Activities of N-ferrocenylmethyl-2- and -3-nitroaniline and Determination of their Binding Parameters with Superoxide Anion Radicals, Current Pharmaceutical Analysis 2017; 13 (2) . https://dx.doi.org/10.2174/1573412912666160831145524

DOI https://dx.doi.org/10.2174/1573412912666160831145524	Print ISSN 1573-4129
Publisher Name Bentham Science Publisher	Online ISSN 1875-676X