Anticancer, Antioxidant and Cytotoxic Potential of Thymol in vitro Brain Tumor Cell Model

Title:Anticancer, Antioxidant and Cytotoxic Potential of Thymol in vitro Brain Tumor Cell Model

Volume: 17 Issue: 2

Author(s): Elanur Aydın*, Hasan Turkez, Sener Tasdemir and Fazıl Hacımuftuoglu

Affiliation:

• Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum,Turkey

Keywords: Antiproliferative, comet assay, MTT assay, N2a neuroblastoma, thymol, total antioxidant capacity.

Abstract: Background: Thymol (THY), which is a monocyclic monoterpene, found in oil of thyme various other kinds of plants. Until today, although different biological properties of THY have been indicated, its neurological toxicity has never been investigated.

Method: In this study, in vitro antiproliferative (by 3-(4,5 dimetylthiazol-2-yl)-2,5 diphenlytetrazolium bromide (MTT) test), genotoxic (by single cell gel electrophoresis (SCGE)) and oxidative effects (by total antioxidant capacity (TAC) and total oxidative status (TOS) analysis) of THY (0-400 mg/L) were assessed on cultured primary rat neurons (CPRNs) and N2a neuroblastoma cells.

Results: The obtained data from MTT analysis revealed that THY (only at 400 mg/L) led to significant (p<0.05) decreases of the cell viability in cultured primary rat neurons. And, THY was found to inhibit cell growth in N2a cells at concentrations of 200 and 400 mg/L. Again, DNA damage rates were statistically indifferent (p>0.05) in both treated cell type as compared to control group. The present results also showed that 10, 25 and 50 mg/L of THY application into the cell cultures supported antioxidant capacity in primary rat neurons but not in N2a cells.

Conclusion: In a conclusion, these results confirm that THY may have antiproliferative potential against brain tumor cells involving oxidative alteration.

Cite this article as:

Aydın Elanur*, Turkez Hasan, Tasdemir Sener and Hacımuftuoglu Fazıl, Anticancer, Antioxidant and Cytotoxic Potential of Thymol in vitro Brain Tumor Cell Model, Central Nervous System Agents in Medicinal Chemistry 2017; 17 (2) . https://dx.doi.org/10.2174/1871524916666160823121854

DOI https://dx.doi.org/10.2174/1871524916666160823121854	Print ISSN 1871-5249
Publisher Name Bentham Science Publisher	Online ISSN 1875-6166