Cell Line and Augument Cellular Uptake Study of Statistically Optimized Sustained Release Capecitabine Loaded Eudragit S100/PLGA(poly(lacticco- glycolic acid)) Nanoparticles for Colon Targeting

Title:Cell Line and Augument Cellular Uptake Study of Statistically Optimized Sustained Release Capecitabine Loaded Eudragit S100/PLGA(poly(lacticco- glycolic acid)) Nanoparticles for Colon Targeting

Volume: 14 Issue: 6

Author(s): Sonia Pandey*, Sanganala Mattha Vijayendra Swamy, Udhshu Mansha Ubaid Ulla, Arti Gupta, Hetal Patel and Jitendra Singh Yadav

Affiliation:

• Department of Pharmaceutics, Maliba Pharmacy College, Bardoli Mahuva Road, Dist. Surat, Gujarat 394350,India

Keywords: Cytotoxicity, controlled release, capecitabine, factorial design, nanoparticles, targeting.

Abstract: Background: Capecitabine, an anti cancer drug, has a very short drug elimination half-life (0.49 to 0.89 h). High doses and absence of targeting ability in the colon region may lead to more side effects to the patients with colon cancer.

Purpose: To develop and optimize sustained release nanoparticles for effective treatment of colon cancer.

Methods: Eudragit S100-PLGA(poly (lactic-co-glycolic acid)) nanoparticles were prepared by a double emulsification, solvent evaporation method followed by high-pressure homogenisation evaluated and the particles were evaluated for surface morphology, particle size analysis, polydispersity index, drug content, % entrapment efficiency and in vitro drug release. To optimize the batch a 32 full factorial design was applied. The optimized batch was evaluated for cytotoxicity and cellular uptake study.

Results and Discussion: The optimized formulation exhibited 179.25 nm mean particle size, 71.27% of drug entrapment efficiency and 81.824% drug release up to 72 h. When the concentration of capecitabine was increased from 50-500 μg/ml, the % cytotoxicity of nanoparticles and capecitabine (pure drug) increased from 8.5 to 97.70% and 2.7 to 82.23%, respectively. As per a cellular uptake study, the optimized nanoparticles were completely uptaken by HT 29 adenocarcinoma cells within 2 to 4 h.

Conclusion: Optimized Eudragit S100-PLGA nanoparticles are a promising delivery system for colon targeting.

Cite this article as:

Pandey Sonia*, Vijayendra Swamy Mattha Sanganala , Ubaid Ulla Mansha Udhshu, Gupta Arti, Patel Hetal and Yadav Singh Jitendra, Cell Line and Augument Cellular Uptake Study of Statistically Optimized Sustained Release Capecitabine Loaded Eudragit S100/PLGA(poly(lacticco- glycolic acid)) Nanoparticles for Colon Targeting, Current Drug Delivery 2017; 14 (6) . https://dx.doi.org/10.2174/1567201813666160817150621

DOI https://dx.doi.org/10.2174/1567201813666160817150621	Print ISSN 1567-2018
Publisher Name Bentham Science Publisher	Online ISSN 1875-5704