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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Research Article

Polyacrylamide Gel-Entrapped Fungal Manganese Peroxidase from Ganoderma lucidum IBL-05 with Enhanced Catalytic, Stability, and Reusability Characteristics

Author(s): Muhammad Bilal, Muhammad Asgher and Hafiz M. N. Iqbal

Volume 23, Issue 9, 2016

Page: [812 - 818] Pages: 7

DOI: 10.2174/0929866523999160722145740

Price: $65

Abstract

In the present study, polyacrylamide gel (PAG) was utilized as bolster material for the immobilization of in-house extracted and partially purified manganese peroxidase (MnP) through an entrapment technique yielding significant MnP immobilization (87.3±3.3 %) and remarkable stability of the enzyme (37.2±2.4 %) after a storage period of two months at 4°C. The immobilization also increased the optimal temperature by 10 °C and provided an alkaline shift of the pH optimum. Moreover, a significant enhancement in the thermo-stability was observed. After an incubation period of 72 h at 50°C, the PAG-entrapped-MnP still exhibited 41.2 % of the initial activity, whereas the free enzyme was completely inactive. Furthermore, PAG-entrapped-MnP showed an excellent recycling efficiency and retained more than 50% of its initial activity after five consecutive reaction cycles. In conclusion, owing to the economic feasibility, carrier-supported MnP may be a promising candidate for various applications in different industrial sectors.

Keywords: Catalytic stability, enzyme immobilization, Ganoderma lucidum, manganese peroxidase, reusability.

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