Optimization of Analytical Strategy for Determination of Total Antimony in Fish Muscle Tissue using Hydride Generation Atomic Absorption Spectrometry

Title:Optimization of Analytical Strategy for Determination of Total Antimony in Fish Muscle Tissue using Hydride Generation Atomic Absorption Spectrometry

Volume: 13 Issue: 4

Author(s): Mariio M. Silva Junior, Danilo J. Leao, Laiana O.B. Silva, Catilea Franca Pimentel, Karina Santos Garcia and Sergio L.C. Ferreira*

Affiliation:

• Instituto de Química, Universidade Federal da Bahia, Salvador, Bahia,Brazil

Keywords: Antimony, fish muscle, HG AAS, experimental design, todos os santos bay, atomic absorption spectrometry, organic species.

Abstract: Background: Antimony is a metaloide that offers human health risks. Then, the determination of this chemical element in food samples is always opportune. This paper proposes a method for determination of total antimony in fish muscle tissue using hydride generation atomic absorption spectrometry (HG AAS).

Methods: The optimization of the quantification step was performed using a two-level full factorial design and a Box Behnken matrix, involving the factors: nitric acid concentration, carrier gas flow (argon) and sodium borohydride concentration. The digestion procedure of the samples was established employing microwave assisted radiation using nitric acid.

Results: The method using the optimized conditions allows the determination of total antimony by the external calibration technique with limits of detection and quantification of 0.8 and 2.6 ng g-1 for a sample mass of 0.20 g, and precision expressed as relative standard deviation of 4.32%, for an antimony solution of concentration 5 μg L-1. The accuracy was confirmed by analysis of a certified reference material of oyster tissue furnished by National Institute of Standards and Technology (NIST).

Conclusion: The method was applied for determination of total antimony in seven samples of fish muscle tissue (Arraia - Brazilian name, Elasmobranquiomorpha rajiformes) acquired in two regions of the Todos os Santos Bay, Bahia, Brazil. The antimony content found varied from 62 to 370 ng g-1. These results are agreeing with other data reported by literature.

Cite this article as:

Silva Junior M. Mariio, Leao J. Danilo, Silva O.B. Laiana, Pimentel Franca Catilea, Garcia Santos Karina and Ferreira L.C. Sergio*, Optimization of Analytical Strategy for Determination of Total Antimony in Fish Muscle Tissue using Hydride Generation Atomic Absorption Spectrometry, Current Analytical Chemistry 2017; 13 (4) . https://dx.doi.org/10.2174/1573411012666160606171414

DOI https://dx.doi.org/10.2174/1573411012666160606171414	Print ISSN 1573-4110
Publisher Name Bentham Science Publisher	Online ISSN 1875-6727