Development of SSR Markers Linked to Low Hydrocyanic Acid Content in Sorghum-Sudan Grass Hybrid Based on BSA Method

Title:Development of SSR Markers Linked to Low Hydrocyanic Acid Content in Sorghum-Sudan Grass Hybrid Based on BSA Method

Volume: 23 Issue: 5

Author(s): Yu Xiao-Xia, Liu Zhi-Hua, Yu Zhuo, Shi Yue and Li Xiao-Yu

Affiliation:

Keywords: Bulked segregant analysis, Hydrocyanic acid, Single marker analysis, Sorghum-Sudan grass hybrid, SSR markers.

Abstract: Sorghum-Sudan grass hybrid containing high hydrocyanic acid content can cause hydrocyanic acid poisoning to the livestock and limit the popularization of this forage crop. Molecular markers associated with low hydrocyanic acid content can speed up the process of identification of genotypes with low hydrocyanic acid content. In the present study, 11 polymorphic SSR primers were screened and used for bulked segregant analysis and single marker analysis. Three SSR markers Xtxp7₂₃₀, Xtxp7₃₇₅ and Bnlg667₉₆₀ associated with low hydrocyanic acid content were rapidly identified by BSA. In single marker analysis, six markers Xtxp7230, Xtxp7375, Bnlg667960, Xtxp67-11, Xtxp295-7 and Xtxp12-9 were linked to low hydrocyanic acid content, which explained the proportion of phenotypic variation from 7.6 % to 41.2 %. The markers identified by BSA were also verified by single marker analysis. The three SSR marker bands were then cloned and sequenced for sequence homology analysis in NCBI. It is the first report on the development of molecular markers associated with low hydrocyanic acid content in sorghum- Sudan grass hybrid. These markers will be useful for genetic improvement of low hydrocyanic acid sorghum-Sudan grass hybrid by marker-assisted breeding.

Cite this article as:

Xiao-Xia Yu, Zhi-Hua Liu, Zhuo Yu, Yue Shi and Xiao-Yu Li, Development of SSR Markers Linked to Low Hydrocyanic Acid Content in Sorghum-Sudan Grass Hybrid Based on BSA Method, Protein & Peptide Letters 2016; 23 (5) . https://dx.doi.org/10.2174/0929866523666160322153559