Abstract
Coupling of a macromolecule such as an enzyme or a receptor to a chromatographic support has revealed a powerful analytical tool to face different aspects related to drug discovery. Chromatographic systems with immobilized macromolecules have been developed to be used for different applications in pharmaceutical analysis including enzyme inhibitor screening, biopharmaceutics structural analysis, chiral separations and binding studies. These areas of research are very challenging both for the technological and for the applicative aspects. This review offers an overview of general strategies and references that can be considered in order to develop innovative and reliable “protein-labs” for specific separative analytical applications of pharmaceutical interest.
Keywords: Protein immobilization, bio-affinity chromatography, immobilized enzyme reactors, chiral stationary phases, drug binding, enantioseparation, inhibitor potency determination.
Current Organic Chemistry
Title:Protein-Labs on Separative Analytical Scale in Medicinal Chemistry: from the Proof of Concept to Applications
Volume: 20 Issue: 11
Author(s): Caterina Temporini, Enrica Calleri, Gloria Brusotti and Gabriella Massolini
Affiliation:
Keywords: Protein immobilization, bio-affinity chromatography, immobilized enzyme reactors, chiral stationary phases, drug binding, enantioseparation, inhibitor potency determination.
Abstract: Coupling of a macromolecule such as an enzyme or a receptor to a chromatographic support has revealed a powerful analytical tool to face different aspects related to drug discovery. Chromatographic systems with immobilized macromolecules have been developed to be used for different applications in pharmaceutical analysis including enzyme inhibitor screening, biopharmaceutics structural analysis, chiral separations and binding studies. These areas of research are very challenging both for the technological and for the applicative aspects. This review offers an overview of general strategies and references that can be considered in order to develop innovative and reliable “protein-labs” for specific separative analytical applications of pharmaceutical interest.
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Temporini Caterina, Calleri Enrica, Brusotti Gloria and Massolini Gabriella, Protein-Labs on Separative Analytical Scale in Medicinal Chemistry: from the Proof of Concept to Applications, Current Organic Chemistry 2016; 20 (11) . https://dx.doi.org/10.2174/1385272819666150810220825
DOI https://dx.doi.org/10.2174/1385272819666150810220825 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
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