Abstract
This paper presents a new disposable electrochemical biosensor for the analysis of Haptoglobin (Hp) which shows many important functionalities such as antioxidant capabilities, antiinflammatory activities and regulation of the immune system. Aminopropyltriethoxysilane (APTES) was self-assembled onto an indium tin oxide (ITO) substrate to fabricate a disposable biosensor. Anti- Hp antibodies were immobilized onto the modified ITO surface covalently. The optimization of the construction parameters and the analytical performance of the biosensor were examined and compared. The methods, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (EIS) were operated for detailed characterization of the different surfaces of the biosensor. Kramers-Kronig Transform was also applied to raw EIS data for checking the verification of obtained experimental EIS data. Moreover, a useful method, single frequency impedance (SFI), was successfully operated to characterize the binding of Hp to the electrode surface modified with anti-Hp. At last, the presented biosensor was applied to the analysis of artificial serum samples, which were spiked with Hp.
Keywords: Haptoglobin, indium tin oxide, biosensor, acute phase protein, electrochemical impedance spectroscopy, single frequency impedance.
Current Analytical Chemistry
Title:A Disposable, Highly Sensitive Biosensing System: Determination of Haptoglobin as a Significant Acute Phase Biomarker
Volume: 12 Issue: 1
Author(s): Münteha N. Sonuç and Mustafa K. Sezgintürk
Affiliation:
Keywords: Haptoglobin, indium tin oxide, biosensor, acute phase protein, electrochemical impedance spectroscopy, single frequency impedance.
Abstract: This paper presents a new disposable electrochemical biosensor for the analysis of Haptoglobin (Hp) which shows many important functionalities such as antioxidant capabilities, antiinflammatory activities and regulation of the immune system. Aminopropyltriethoxysilane (APTES) was self-assembled onto an indium tin oxide (ITO) substrate to fabricate a disposable biosensor. Anti- Hp antibodies were immobilized onto the modified ITO surface covalently. The optimization of the construction parameters and the analytical performance of the biosensor were examined and compared. The methods, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (EIS) were operated for detailed characterization of the different surfaces of the biosensor. Kramers-Kronig Transform was also applied to raw EIS data for checking the verification of obtained experimental EIS data. Moreover, a useful method, single frequency impedance (SFI), was successfully operated to characterize the binding of Hp to the electrode surface modified with anti-Hp. At last, the presented biosensor was applied to the analysis of artificial serum samples, which were spiked with Hp.
Export Options
About this article
Cite this article as:
Sonuç N. Münteha and Sezgintürk K. Mustafa, A Disposable, Highly Sensitive Biosensing System: Determination of Haptoglobin as a Significant Acute Phase Biomarker, Current Analytical Chemistry 2016; 12 (1) . https://dx.doi.org/10.2174/1573411011666150603201229
DOI https://dx.doi.org/10.2174/1573411011666150603201229 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Genetic Polymorphisms of Drug Metabolizing Enzymes and Transporters: The Long Way from Bench to Bedside
Current Topics in Medicinal Chemistry Ticagrelor Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Rats by the Inhibition of TGF-β1/Smad3 and PI3K/AKT/mTOR Pathways
Current Molecular Pharmacology Antioxidant Potential of Solvent Partitioned Extraction from Aqueous Extract of Gracilaria Tenuistipitata
Current Organic Chemistry The Role of DNA-Microarray in Translational Cancer Research
Current Pharmacogenomics Nanomedicines Based Drug Delivery Systems for Anti-Cancer Targeting and Treatment
Current Drug Delivery Recent Developments of Targeted Therapies in the Treatment of Non-Small Cell Lung Cancer
Current Drug Discovery Technologies The Biological Effects of Diagnostic Cardiac Imaging
Current Pharmaceutical Design Dual-Specificity MAP Kinase Phosphatases as Targets of Cancer Treatment
Anti-Cancer Agents in Medicinal Chemistry Microbial Efflux Pump Inhibition: Tactics and Strategies
Current Pharmaceutical Design To Be, or Not To Be: That is the Question-Lineage Commitment in Hematopoiesis
Current Immunology Reviews (Discontinued) Current Treatment Strategies for Multiple Sclerosis - Efficacy Versus Neurological Adverse Effects
Current Pharmaceutical Design Subject Index To Volume 2
Current Medicinal Chemistry - Anti-Cancer Agents The Role of the Antiangiogenetic Ramucirumab in the Treatment of Advanced Non Small Cell Lung Cancer
Current Medicinal Chemistry Epigenetics in Cystic Fibrosis: Epigenetic Targeting of a Genetic Disease
Current Drug Targets Pharmacogenomics in Drug Induced Liver Injury
Current Drug Metabolism Features Identification for Phenotypic Classification Based on Genes and Gene Pairs
Current Bioinformatics PI3K/Akt Signalling Pathway Specific Inhibitors: A Novel Strategy to Sensitize Cancer Cells to Anti-Cancer Drugs
Current Pharmaceutical Design Exploration of Metastasis-related Proteins as Biomarkers and Therapeutic Targets in the Treatment of Head and Neck Cancer
Current Cancer Drug Targets Quantitative Determination and Validation of Four Phenolic Acids in <i>Salvia Miltiorrhiza</i> Bunge using <sup>1</sup>H-NMR Spectroscopy
Current Pharmaceutical Analysis Chemical Structure and Surface Modification of Dendritic Nanomaterials Tailored for Therapeutic and Diagnostic Applications
Current Topics in Medicinal Chemistry