Abstract
In this study, we demonstrated an oxidative method with free radical to generate 3,5,4-trihydroxy-transstilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI–MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4-dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of its in situ generation of oxidative derivatives followed by LC-ESI–MS/MS can be utilized to evaluate different metabolites in various conditions.
Keywords: Free radical, metabolite, resveratrol, ammonium persulfate (APS), liquid chromatography tandem mass spectrometry (LC–MS/MS), multiple reaction monitoring (MRM).
Current Analytical Chemistry
Title:A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry
Volume: 11 Issue: 4
Author(s): Wangta Liu, Yow-Ling Shiue, Yi-Reng Lin, Hugo You-Hsien Lin and Shih-Shin Liang
Affiliation:
Keywords: Free radical, metabolite, resveratrol, ammonium persulfate (APS), liquid chromatography tandem mass spectrometry (LC–MS/MS), multiple reaction monitoring (MRM).
Abstract: In this study, we demonstrated an oxidative method with free radical to generate 3,5,4-trihydroxy-transstilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI–MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4-dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of its in situ generation of oxidative derivatives followed by LC-ESI–MS/MS can be utilized to evaluate different metabolites in various conditions.
Export Options
About this article
Cite this article as:
Liu Wangta, Shiue Yow-Ling, Lin Yi-Reng, Lin You-Hsien Hugo and Liang Shih-Shin, A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry, Current Analytical Chemistry 2015; 11 (4) . https://dx.doi.org/10.2174/1573411011666150515233817
DOI https://dx.doi.org/10.2174/1573411011666150515233817 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Recent Developments in Polymeric Nanoparticle Engineering and Their Applications in Experimental and Clinical Oncology
Anti-Cancer Agents in Medicinal Chemistry Pharmacokinetic Profiles of Anticancer Herbal Medicines in Humans and the Clinical Implications
Current Medicinal Chemistry Novel Spiro/non-Spiro Pyranopyrazoles: Eco-Friendly Synthesis, In-vitro Anticancer Activity, DNA Binding, and In-silico Docking Studies
Current Bioactive Compounds Microcavity Plasma Devices
Recent Patents on Engineering Phytotherapeutic Agents for Benign Prostatic Hyperplasia: An Overview
Mini-Reviews in Medicinal Chemistry Mathematical Methods to Analysis of Topology, Functional Variability and Evolution of Metabolic Systems Based on Different Decomposition Concepts
Current Drug Metabolism Perfluoroalkylsulfonyloxyisoflavones: Synthesis and Anti-Proliferation of HL-60
Letters in Organic Chemistry Isothiocyanates in the Chemoprevention of Bladder Cancer
Current Drug Metabolism Computational Models for Predicting Interactions with Cytochrome p450 Enzyme
Current Topics in Medicinal Chemistry Therapeutic Applications of Mesenchymal Stem Cells: A Comprehensive Review
Current Stem Cell Research & Therapy Drug Repurposing: An Emerging Tool for Drug Reuse, Recycling and Discovery
Current Drug Research Reviews A DNA Repair Pathway Polymorphism (rs25487) and Angiographically Proven Coronary Artery Patients in a Population of Southern Iran
Cardiovascular & Hematological Agents in Medicinal Chemistry Procarbazine – A Traditional Drug in the Treatment of Malignant Gliomas
Current Medicinal Chemistry Synthesis of 2-Methyl N10-Substituted Acridones as Selective Inhibitors of Multidrug Resistance (MDR) Associated Protein in Cancer Cells
Medicinal Chemistry Osteoporotic Fracture Healing: Potential Use of Medicinal Plants from the Tropics
Current Drug Targets Inflammatory Bowel Disease: Etiology, Pathogenesis and Current Therapy
Current Pharmaceutical Design Natural Compounds with Proteasome Inhibitory Activity for Cancer Prevention and Treatment
Current Protein & Peptide Science Identification and Determination of Potential Impurities Present in the Acyclovir Drug Substance by GC-MS
Current Pharmaceutical Analysis Atmospheric Concentrations of Polycyclic Aromatic Hydrocarbons (PAHs) in India and South Korea: A Review
Mini-Reviews in Organic Chemistry Synthesis of New 3-Cyanocoumarins with C-6 Azo Function Using Ultrasound and Grinding Techniques in the Presence of Nano Fe<sub>3</sub>O<sub>4</sub>
Letters in Organic Chemistry