Abstract
The vasodilatation effects of four components in Baige capsule were investigated on isolated cerebral basilar arteries and mesenteric arteries model of rats. In order to inspect the potential mechanism, the SH-SY5Y cellular membrane chromatography (CMC) was established to specially recognize the ligand which could interact with N-methyl-D-aspartate receptor (NMDA). Frontal chromatography and competitive binding chromatography were applied to investigate the interaction of four main components in Baige capsule with this receptor. MK-801 (an antagonist of NMDA receptor) was used as the displacer, ketamine hydrochloride, puerarin, daidzein, imperatorin, and isoimperatorin was used as the ligand. The results of vasodilatation test suggested that imperatorin and isoimperatorin could effectively relax both of the cerebral basilar arteries and mesenteric arteries. The CMC study showed that imperatorin and isoimperatorin could competitively displace MK-801. The molecular docking model of the NMDA receptor indicated that imperatorin and isoimperatorin showed a similar docking behavior as MK-801. Overall, the studies demonstrated that imperatorin and isoimperatorin had obvious vasodilatation effect and might be prospective candidates as new NMDA receptor antagonists.
Keywords: Cellular membrane chromatography, MK-801, NMDA, vasodilation, imperatorin, puerarin.
Current Pharmaceutical Analysis
Title:The Vasodilatation Effects of Four Active Components in Baige Capsule and the Potential Mechanism of Them with the NMDA Receptor
Volume: 11 Issue: 3
Author(s): Yuanji Wang, Yanmin Zhang, Weina Ma, Xiaoyan Pan, Nan Zhou, Bingling Dai and Langchong He
Affiliation:
Keywords: Cellular membrane chromatography, MK-801, NMDA, vasodilation, imperatorin, puerarin.
Abstract: The vasodilatation effects of four components in Baige capsule were investigated on isolated cerebral basilar arteries and mesenteric arteries model of rats. In order to inspect the potential mechanism, the SH-SY5Y cellular membrane chromatography (CMC) was established to specially recognize the ligand which could interact with N-methyl-D-aspartate receptor (NMDA). Frontal chromatography and competitive binding chromatography were applied to investigate the interaction of four main components in Baige capsule with this receptor. MK-801 (an antagonist of NMDA receptor) was used as the displacer, ketamine hydrochloride, puerarin, daidzein, imperatorin, and isoimperatorin was used as the ligand. The results of vasodilatation test suggested that imperatorin and isoimperatorin could effectively relax both of the cerebral basilar arteries and mesenteric arteries. The CMC study showed that imperatorin and isoimperatorin could competitively displace MK-801. The molecular docking model of the NMDA receptor indicated that imperatorin and isoimperatorin showed a similar docking behavior as MK-801. Overall, the studies demonstrated that imperatorin and isoimperatorin had obvious vasodilatation effect and might be prospective candidates as new NMDA receptor antagonists.
Export Options
About this article
Cite this article as:
Wang Yuanji, Zhang Yanmin, Ma Weina, Pan Xiaoyan, Zhou Nan, Dai Bingling and He Langchong, The Vasodilatation Effects of Four Active Components in Baige Capsule and the Potential Mechanism of Them with the NMDA Receptor, Current Pharmaceutical Analysis 2015; 11 (3) . https://dx.doi.org/10.2174/1573412911666150106235124
DOI https://dx.doi.org/10.2174/1573412911666150106235124 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Chemopreventive Properties and Molecular Mechanisms of the Bioactive Compounds in Hibiscus Sabdariffa Linne
Current Medicinal Chemistry COX-2 Signaling and Cancer: New Players in Old Arena
Current Drug Targets Mechanical Circulatory Support in Children
Current Cardiology Reviews Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase
Protein & Peptide Letters The Gastrin-Releasing Peptide Receptor as a Therapeutic Target in Central Nervous System Disorders
Recent Patents on CNS Drug Discovery (Discontinued) Effect of Solvent Type, Extraction Temperature, Agitation Speed and Microwave Power on Phenolic Compound Extraction and Antioxidant Activity of Apricot Kernels (<i>Prunus armeniaca</i> L.)
Current Bioactive Compounds Molecular Mechanisms of Inherited Arrhythmias
Current Genomics Circulating Biochemical Markers of Brain Damage in Infants Complicated by Ischemia Reperfusion Injury
Cardiovascular & Hematological Agents in Medicinal Chemistry Adenosine and Adenosine 5’-triphosphate Catabolism in Systemic Blood as a Potential Biomarker for Doxorubicin Cardiotoxicity in an Experimental Rat Model in vivo
Cardiovascular & Hematological Disorders-Drug Targets Peptide Therapeutics in Neurodegenerative Disorders
Current Medicinal Chemistry Pharmacological and Non Pharmacological Strategies in the Management of Coronary Artery Disease and Chronic Kidney Disease
Current Cardiology Reviews Specific Therapy Based on the Genotype and Cellular Mechanism in Inherited Cardiac Arrhythmias. Long QT Syndrome and Brugada Syndrome
Current Pharmaceutical Design Epidemiology of Diabetes Mellitus in the United Arab Emirates
Current Diabetes Reviews A Network Biology Approach for Assessing the Role of Pathologic Adipose Tissues in Insulin Resistance Using Meta-analysis of Microarray Datasets
Current Genomics Evaluation of Vancoplus Versus Ceftriaxone Against Cephalosporin Resistance MRSA Strain in Experimental Meningitis Model
Cardiovascular & Hematological Disorders-Drug Targets Leukocyte-mediated Tissue Injury in Ischemic Stroke
Current Medicinal Chemistry Innovative Concepts of Cell Therapy: Pluripotent and Multipotent Stem Cells and New Bio-Material Solution in Research and Clinical Application
Recent Patents on Regenerative Medicine Development of Tablet Device App for Parkinson’s Disease Patients’ Continuous Self-Monitoring and Management
Neuroscience and Biomedical Engineering (Discontinued) Targeting TNF-Alpha to Elucidate and Ameliorate Neuroinflammation in Neurodegenerative Diseases
CNS & Neurological Disorders - Drug Targets Ifenprodil, a Novel NMDA Receptor Antagonist : Site and Mechanism of Action
Current Drug Targets