Abstract
The present investigation describes two simple, sensitive and validated spectrophotometric methods for the determination of Cyclophosphamide (CP) in pure and formulated forms. The methods are mainly based on the formation of charge transfer (CT) complex, with the reagents 2,3-dichloro-5,6- dicyano-1,4-benzoquinone (DDQ) (Method A) and p-Chloranilic acid (PCA) (Method B), hence a stable, 1:1 stoichiometric complex was obtained, which shows orangeyellow and pink colors, respectively. The produced chromogens optical densities were measured at 352 and 522 nm, respectively. The Beer’s law was tested in the range of 2-16 and 5-50 μg mL-1. The optical characteristics such as molar absorptivity (ɛ), and Sandell’s sensitivity values were calculated. Other analytical parameters like, Limit of Detection (LOD), Limit of Quantification (LOQ), accuracy and precisions were evaluated. The proposed methods were successfully applied for the determination of the CP in tablet formulation with higher accuracy, better recoveries and less than 2% relative standard deviation.
Keywords: Cyclophosphamide, charge transfer complex, DDQ, p-chloranilic acid, spectrophotometry, validation.
Current Pharmaceutical Analysis
Title:Application of Charge Transfer Reactions for the Quantitative Spectrophotometric Determination of Cyclophosphamide in Pure and Pharmaceutical Formulation
Volume: 11 Issue: 2
Author(s): K. Siddappa and Prashant C. Hanamshetty
Affiliation:
Keywords: Cyclophosphamide, charge transfer complex, DDQ, p-chloranilic acid, spectrophotometry, validation.
Abstract: The present investigation describes two simple, sensitive and validated spectrophotometric methods for the determination of Cyclophosphamide (CP) in pure and formulated forms. The methods are mainly based on the formation of charge transfer (CT) complex, with the reagents 2,3-dichloro-5,6- dicyano-1,4-benzoquinone (DDQ) (Method A) and p-Chloranilic acid (PCA) (Method B), hence a stable, 1:1 stoichiometric complex was obtained, which shows orangeyellow and pink colors, respectively. The produced chromogens optical densities were measured at 352 and 522 nm, respectively. The Beer’s law was tested in the range of 2-16 and 5-50 μg mL-1. The optical characteristics such as molar absorptivity (ɛ), and Sandell’s sensitivity values were calculated. Other analytical parameters like, Limit of Detection (LOD), Limit of Quantification (LOQ), accuracy and precisions were evaluated. The proposed methods were successfully applied for the determination of the CP in tablet formulation with higher accuracy, better recoveries and less than 2% relative standard deviation.
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Siddappa K. and C. Hanamshetty Prashant, Application of Charge Transfer Reactions for the Quantitative Spectrophotometric Determination of Cyclophosphamide in Pure and Pharmaceutical Formulation, Current Pharmaceutical Analysis 2015; 11 (2) . https://dx.doi.org/10.2174/1573412910666141024215724
DOI https://dx.doi.org/10.2174/1573412910666141024215724 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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