Partitioning of Invertase in Nanoparticle Incorporated Aqueous Twophase Systems

Title:Partitioning of Invertase in Nanoparticle Incorporated Aqueous Twophase Systems

Volume: 1 Issue: 2

Author(s): MC Madhusudhan, A Hrishikesh Tavanandi and KSMS Raghavarao

Affiliation:

Keywords: Aqueous two phase extraction, invertase, partition coefficient, nanoparticles, purification, recovery.

Abstract: Partitioning studies of invertase are carried out by incorporating different nanoparticles at varied concentrations (0-2 %, w/w) employing polymer/polymer (PEG-3350/dextran-T70) and polymer/salt (PEG-3350/magnesium sulphate) phase systems. The partition coefficient of total protein (K_TP) is found to decrease in case of PEG-3350/dextran-T70 system with an increase in concentration of nanoparticles. However, the extent of decrease varied depending on the nanoparticle. Significant changes are not observed in partition coefficient of invertase (K_INV). In case of PEG- 3350/magnesium sulphate systems, gradual increase in K_TP and K_INV values is observed with an increase in concentration of nanoparticles. The partition coefficient varied (K_TP from 0.06 to 0.25 and K_INV from 0.01 to 0.04) depending on the nanoparticles. Highest enrichment of enzyme is observed in case of PEG-3350/magnesium sulphate system with gold nanoparticles (32.64 U/mg specific activity with 9 fold enrichment and enzyme activity recovery of 92%) among all the systems studied.

Cite this article as:

Madhusudhan MC, Tavanandi Hrishikesh A and Raghavarao KSMS, Partitioning of Invertase in Nanoparticle Incorporated Aqueous Twophase Systems, Current Biochemical Engineering (Discontinued) 2014; 1 (2) . https://dx.doi.org/10.2174/2212711901666140515004044