Abstract
A new, simple HPLC method is described for the isocratic separation of biogenic monoamines, in milk samples, in the presence of hexylamine as the internal standard, after pre-column derivatization with naphthalene-2,3- dicarboxaldehyde /cyanide ions. The derivatization reaction variables including buffer pH and concentration, reaction time, naphthalene-2,3-dicarboxaldehyde (NDA) and cyanide concentrations were optimized. The isoindoles formed were eluted by means of methanol/water (80:20, v/v), at a flow rate of 1 mL min-1, on an Inertsil ODS-3 column (250 x 4 mm i.d., 5 µm), in less than 30 min. Fluorometric detection was used, at excitation and emission wavelengths of 424 and 494 nm, respectively. The limits of detection were in the pg level (7 – 80 pg) using a 10 µL-loop. The whole procedure was evaluated and fully validated for the determination of biogenic amines in milk samples, after ultrasound-assisted liquidliquid extraction. The intra-assay precision and accuracy were calculated in samples spiked with different levels of biogenic amines and yielded RSD and recovery values in the range 0.3 – 4.2% and 82.5 – 103.8% respectively.
Keywords: Biogenic amines, derivatization, fluorescence, HPLC, milk, naphthalene-2, 3-dicarboxaldehyde.
Current Analytical Chemistry
Title:Assay of Biogenic Monoamines in Milk by Fluorescence-LC Following Derivatization with Naphthalene-2,3-dicarboxaldehyde
Volume: 10 Issue: 3
Author(s): Maria Notou and Anastasia Zotou
Affiliation:
Keywords: Biogenic amines, derivatization, fluorescence, HPLC, milk, naphthalene-2, 3-dicarboxaldehyde.
Abstract: A new, simple HPLC method is described for the isocratic separation of biogenic monoamines, in milk samples, in the presence of hexylamine as the internal standard, after pre-column derivatization with naphthalene-2,3- dicarboxaldehyde /cyanide ions. The derivatization reaction variables including buffer pH and concentration, reaction time, naphthalene-2,3-dicarboxaldehyde (NDA) and cyanide concentrations were optimized. The isoindoles formed were eluted by means of methanol/water (80:20, v/v), at a flow rate of 1 mL min-1, on an Inertsil ODS-3 column (250 x 4 mm i.d., 5 µm), in less than 30 min. Fluorometric detection was used, at excitation and emission wavelengths of 424 and 494 nm, respectively. The limits of detection were in the pg level (7 – 80 pg) using a 10 µL-loop. The whole procedure was evaluated and fully validated for the determination of biogenic amines in milk samples, after ultrasound-assisted liquidliquid extraction. The intra-assay precision and accuracy were calculated in samples spiked with different levels of biogenic amines and yielded RSD and recovery values in the range 0.3 – 4.2% and 82.5 – 103.8% respectively.
Export Options
About this article
Cite this article as:
Notou Maria and Zotou Anastasia, Assay of Biogenic Monoamines in Milk by Fluorescence-LC Following Derivatization with Naphthalene-2,3-dicarboxaldehyde, Current Analytical Chemistry 2014; 10 (3) . https://dx.doi.org/10.2174/1573411010999131219103035
DOI https://dx.doi.org/10.2174/1573411010999131219103035 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
Biogeochemistry: global environmental research and management
his issue focuses on the bio-pathways of trace elements (Ni, Co, Cr, Mn, Cd, Pb, Zn and REE) in soil, plant and atmospheric systems. Specific interest in the application of phytotechnologies that utilise hyperaccumulator plants (agro/phytomining). On the basis of understanding the migration and transformation of trace elements in ecosystem, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
From Clinical Application to Cognitive Enhancement: The Example of Methylphenidate
Current Neuropharmacology Hypersexuality As a Neuropsychiatric Disorder: The Neurobiology and Treatment Options
Current Drug Targets Prophylaxis and Post-exposure Treatment of Intoxications Caused by Nerve Agents and Organophosphorus Pesticides
Mini-Reviews in Medicinal Chemistry A beta oligomerization A Therapeutic Target for Alzheimers Disease
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents Genetic Signatures in Ischemic Stroke: Focus on Aspirin Resistance
CNS & Neurological Disorders - Drug Targets False Resistance to Antiparasitic Drugs: Causes from Shelf Availability to Patient Compliance
Anti-Infective Agents in Medicinal Chemistry Aqueous Humor Pharmacokinetics of Dorzolamide Loaded PLGAChitosan Nanoparticles by Ultra Performance Liquid Chromatography
Current Pharmaceutical Analysis Microwave Energy in Ugi Four-Component Reaction (Ugi-4CR): Expedient Synthesis of Steroid-Peptide Conjugates Based on Aminosteroids - An Important Class of Hybrid Systems
Current Organic Synthesis Synthesis of 2`-hydroxy-4`-isoprenyloxychalcone Derivatives with Potential Antidepressant-like Activity
Medicinal Chemistry Systemic Oxidative Stress in Older Patients with Mild Cognitive Impairment or Late Onset Alzheimer's Disease
Current Alzheimer Research Modulation of Energy Intake and Expenditure Due to Habitual Physical Exercise
Current Pharmaceutical Design A Case of Postpartum Maternal Death with COVID-19 in the West of Iran
Current Women`s Health Reviews Predicting Efficacy and Toxicity in the Era of Targeted Therapy: Focus on Anti-EGFR and Anti-VEGF Molecules
Current Drug Metabolism Benzimidazole Derivatives as Kinase Inhibitors
Current Medicinal Chemistry Editorial [Hot Topic:Nicotinamide Adenine Dinucleotide Biology and Disease(Executive Editor: W. Todd Penberthy)]
Current Pharmaceutical Design Tau Oligomers as Potential Targets for Immunotherapy for Alzheimers Disease and Tauopathies
Current Alzheimer Research Editorial [ Hot Topic:Endocannabinoid-Binding Receptors: Old Friends and New Comers (Guest Editor: Mauro Maccarrone)]
Current Medicinal Chemistry Novel Perspectives in Celiac Disease Therapy
Mini-Reviews in Medicinal Chemistry Renal COX-2, Cytokines and 20-HETE: Tubular and Vascular Mechanisms
Current Pharmaceutical Design Catching the Therapeutic Window of Opportunity in Early Crohn`s Disease
Current Drug Targets