Abstract
Stem cell therapy and research have been a debated topicin recent years. Regardless of the usefulness and great potential of stem cells in the fields of tissue engineering and regenerative medicine, preservation techniques suitable for stem cells have not yet been established. We attempted to develop a preservation solution that can enhance the viability of stem cells after preservation.
Cryopreservation is a standard technique for long-term cell storage, but the toxicity of conventional cryoprotective agents (CPAs) such as dimethylsulfoxide(DMSO) make it difficult to recover cells after thawing. ε-poly-L-lysine (PLL), in which the amino groups were converted to carboxyl groups at an appropriate ratio (more than 50mol % to 80mol %), was shown to have higher cryopreservation efficiency and lower cytotoxicity than current cryoprotective agents (CPAs). Using PLL with 65 mol% [PLL(0.65)] carboxylation, the protective effect on mouse induced pluripotent stem (iPS) cells under hypothermic preservation (4°C and -196°C) was investigated.
After preservation, iPS cells retained their proliferation capacity and expressed markers of undifferentiated cells. Thus, we concluded that an effective and safe CPA was developed. PLL(0.65) can therefore be a valuable component of a DMSO-free, xeno-free, and chemically defined preservation solution for stem cells that also can be utilized at 4°C.
Keywords: Cryopreservation, DMSO-free poly-L-lysine, hypothermic preservation, induced pluripotent stem (iPS) cells.
Current Nanoscience
Title:Hypothermicpreservation of Mouse Induced Pluripotent Stem Cells by Polyampholytes
Volume: 10 Issue: 2
Author(s): Kazuaki Matsumura, HakHee Kim and Suong-Hyu Hyon
Affiliation:
Keywords: Cryopreservation, DMSO-free poly-L-lysine, hypothermic preservation, induced pluripotent stem (iPS) cells.
Abstract: Stem cell therapy and research have been a debated topicin recent years. Regardless of the usefulness and great potential of stem cells in the fields of tissue engineering and regenerative medicine, preservation techniques suitable for stem cells have not yet been established. We attempted to develop a preservation solution that can enhance the viability of stem cells after preservation.
Cryopreservation is a standard technique for long-term cell storage, but the toxicity of conventional cryoprotective agents (CPAs) such as dimethylsulfoxide(DMSO) make it difficult to recover cells after thawing. ε-poly-L-lysine (PLL), in which the amino groups were converted to carboxyl groups at an appropriate ratio (more than 50mol % to 80mol %), was shown to have higher cryopreservation efficiency and lower cytotoxicity than current cryoprotective agents (CPAs). Using PLL with 65 mol% [PLL(0.65)] carboxylation, the protective effect on mouse induced pluripotent stem (iPS) cells under hypothermic preservation (4°C and -196°C) was investigated.
After preservation, iPS cells retained their proliferation capacity and expressed markers of undifferentiated cells. Thus, we concluded that an effective and safe CPA was developed. PLL(0.65) can therefore be a valuable component of a DMSO-free, xeno-free, and chemically defined preservation solution for stem cells that also can be utilized at 4°C.
Export Options
About this article
Cite this article as:
Matsumura Kazuaki, Kim HakHee and Hyon Suong-Hyu, Hypothermicpreservation of Mouse Induced Pluripotent Stem Cells by Polyampholytes, Current Nanoscience 2014; 10 (2) . https://dx.doi.org/10.2174/1573413709999131209123737
DOI https://dx.doi.org/10.2174/1573413709999131209123737 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Novel Chromeno[2,3-d]pyrimidines-Design, Synthesis and Antioxidant Activity
Letters in Drug Design & Discovery Recent Developments in Medicated Lozenges: A Promising Dosage Form for the Effective Delivery of Therapeutic Agents
Drug Delivery Letters Role of MicroRNA 21 in Mesenchymal Stem Cell (MSC) Differentiation: A Powerful Biomarker in MSCs Derived Cells
Current Pharmaceutical Biotechnology Bioactive Medical Coatings for Bone Tissue Engineering
Recent Patents on Engineering Stability Indicating RP-HPLC Method for Analysis of Ketoprofen in Bulk Drug and Eugenol Containing Nanoemulsion Gel (NEG) Formulation Using Response Surface Methodology
Current Pharmaceutical Analysis The Effects of Mesenchymal Stem Cells in Craniofacial Tissue Engineering
Current Stem Cell Research & Therapy Intestinal MicrobiOMICS to Define Health and Disease in Human and Mice
Current Pharmaceutical Biotechnology Therapeutic Potential of Mucoadhesive Drug Delivery Systems - An Updated Patent Review
Recent Patents on Drug Delivery & Formulation Human Beta-Defensin-3: A Promising Antimicrobial Peptide
Mini-Reviews in Medicinal Chemistry Preparation and Properties of Photosensitizer Loaded Magnetic Nanocarriers
Current Nanoscience Enzyme Inhibition as a Key Target for the Development of Novel Metal-Based Anti-Cancer Therapeutics
Anti-Cancer Agents in Medicinal Chemistry Fungal Biofilms and Antimycotics
Current Drug Targets Salivary Genomics, Transcriptomics and Proteomics: The Emerging Concept of the Oral Ecosystem and their Use in the Early Diagnosis of Cancer and other Diseases
Current Genomics Potential Use of Polymeric Nanoparticles for Drug Delivery Across the Blood-Brain Barrier
Current Medicinal Chemistry Nanofibre Based Smart Pharmaceutical Scaffolds for Wound Repair and Regenerations
Current Pharmaceutical Design Effect of Simvastatin on Inflammatory Cytokines Balance in Air Pouch Granuloma Model
Inflammation & Allergy - Drug Targets (Discontinued) Dental Therapeutic Systems
Recent Patents on Drug Delivery & Formulation Introduction: P2 Receptors
Current Topics in Medicinal Chemistry Inflammatory Biomarkers in Chronic Kidney Disease: A Review
Recent Patents on Biomarkers MicroRNA Regulation in Osteogenic and Adipogenic Differentiation of Bone Mesenchymal Stem Cells and its Application in Bone Regeneration
Current Stem Cell Research & Therapy