Abstract
Amphotericin B (AmB) is a poorly water soluble polyene antifungal antibiotic which is negligibly absorbed from the gastro intestinal tract after oral administration. The objective of this research work was to study the oral bioavailability and stability of a self-emulsifying drug delivery system (SEDDS) of amphotericin B (AmB). The SEDDS formulation consisted of glyceryl monooleate, tween 80, polyethylene glycol 400 (PEG 400) and propylene glycol and had AmB content of about 8 mg per ml. The stability of the SEDDS formulation was studied in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) in comparison to pure drug. Oral bioavailability of the SEDDS formulation was studied in rats in comparison to the pure drug. The formulation was filled in two different types of capsule shell, namely HPMC capsule and hard gelatin capsule and stability of the formulation was studied for 3 months. The SEDDS formulation resulted in a mean AUC value of 40.57µg/ml.hr with mean peak plasma concentration of 6.17µg/ml reached after 2 hours after oral administration in rats, whereas concentration of AmB in plasma was not detectable after administration of the pure drug. The formulation filled in hard gelatin capsule shell was physically and chemically stable for more than 3 months under refrigeration (4°C). The study demonstrates that SEDDS approach can be successfully utilized for oral delivery of AmB.
Keywords: Amphotericin B, Oral bioavailability, SEDDS, SGF, SIF, Stability.
Current Drug Delivery
Title:Oral Bioavailability and Stability Study of a Self-Emulsifying Drug Delivery System (SEDDS) of Amphotericin B
Volume: 10 Issue: 5
Author(s): Arundhati Bhattacharyya and Meenakshi Bajpai
Affiliation:
Keywords: Amphotericin B, Oral bioavailability, SEDDS, SGF, SIF, Stability.
Abstract: Amphotericin B (AmB) is a poorly water soluble polyene antifungal antibiotic which is negligibly absorbed from the gastro intestinal tract after oral administration. The objective of this research work was to study the oral bioavailability and stability of a self-emulsifying drug delivery system (SEDDS) of amphotericin B (AmB). The SEDDS formulation consisted of glyceryl monooleate, tween 80, polyethylene glycol 400 (PEG 400) and propylene glycol and had AmB content of about 8 mg per ml. The stability of the SEDDS formulation was studied in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) in comparison to pure drug. Oral bioavailability of the SEDDS formulation was studied in rats in comparison to the pure drug. The formulation was filled in two different types of capsule shell, namely HPMC capsule and hard gelatin capsule and stability of the formulation was studied for 3 months. The SEDDS formulation resulted in a mean AUC value of 40.57µg/ml.hr with mean peak plasma concentration of 6.17µg/ml reached after 2 hours after oral administration in rats, whereas concentration of AmB in plasma was not detectable after administration of the pure drug. The formulation filled in hard gelatin capsule shell was physically and chemically stable for more than 3 months under refrigeration (4°C). The study demonstrates that SEDDS approach can be successfully utilized for oral delivery of AmB.
Export Options
About this article
Cite this article as:
Bhattacharyya Arundhati and Bajpai Meenakshi, Oral Bioavailability and Stability Study of a Self-Emulsifying Drug Delivery System (SEDDS) of Amphotericin B, Current Drug Delivery 2013; 10 (5) . https://dx.doi.org/10.2174/15672018113109990001
DOI https://dx.doi.org/10.2174/15672018113109990001 |
Print ISSN 1567-2018 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5704 |
Call for Papers in Thematic Issues
Advances of natural products, bio-actives and novel drug delivery system against emerging viral infections
Due to the increasing prevalence of viral infections and the ability of these human pathogens to develop resistance to current treatment strategies, there is a great need to find and develop new compounds to combat them. These molecules must have low toxicity, specific activity and high bioavailability. The most suitable ...read more
Electrospun Fibers as Drug Delivery Systems
In recent years, electrospun fibers have attracted considerable attention as potential platforms for drug delivery due to their distinctive properties and adaptability. These fibers feature a notable surface area-to-volume ratio and can be intentionally designed with high porosity, facilitating an increased capacity for drug loading and rendering them suitable for ...read more
Emerging Nanotherapeutics for Mitigation of Neurodegenerative Disorders
Conditions affecting the central nervous system (CNS) present a significant hurdle due to limited access of both treatments and diagnostic tools for the brain. The blood-brain barrier (BBB) acts as a barrier, restricting the passage of molecules from the bloodstream into the brain. The most formidable challenge facing scientists is ...read more
Nanotechnology Based Chemotherapy for the treatment of Head & Neck Cancer
The escalating recurrence rates observed in Head and Neck cancer, particularly within the chemo-therapeutically treated cohort (50-60%), can be attributed to the non-selective nature of current anticancer drug delivery modalities. In this context, nanotechnology-based drug delivery systems emerge as a promising avenue for achieving precise localization of therapeutic agents to ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Impact of CYP2D6 Genetic Variation on the Response of the Cardiovascular Patient to Carvedilol and Metoprolol
Current Drug Metabolism Oxidative stress in carcinogenesis: new synthetic compounds with dual effects upon free radicals and cancer.
Current Medicinal Chemistry Therapeutics Interventions with Anti-Inflammatory Creams in Post Radiation Acute Skin Reactions: A Systematic Review of Most Important Clinical Trials
Recent Patents on Inflammation & Allergy Drug Discovery New Chemoenzymatic Synthesis of (±)-N-(2-hydroxyethyl)-N,N-dime thyl- 2,3-bis(tetradecyloxy)-1-propanammonium Bromide (DMRIE)
Letters in Organic Chemistry Image Fusion of Diagnostic Ultrasound with Other Modalities
Current Medical Imaging Transmucosal Drug Delivery- An Overview
Drug Delivery Letters Resveratrol and Neurodegenerative Diseases: Activation of SIRT1 as the Potential Pathway towards Neuroprotection
Current Neurovascular Research Mass Spectrometric Imaging of the Nervous System
Current Pharmaceutical Design Physcion and Physcion 8-O-β-D-glucopyranoside: Natural Anthraquinones with Potential Anticancer Activities
Current Drug Targets Peptide-Based Nanoparticle for Ex Vivo and In Vivo Dug Delivery
Current Pharmaceutical Design Peptide Agonists and Antagonists with Potential Application in Neurological Disorders
Recent Patents on CNS Drug Discovery (Discontinued) Application of Microfluidics in Single-cell Manipulation, Omics and Drug Development
Current Medicinal Chemistry A Novel Quinazoline-4-one Derivatives as a Promising Cytokine Inhibitors: Synthesis, Molecular Docking, and Structure-activity Relationship
Current Pharmaceutical Biotechnology Stereological Quantification of Blood and Lymph Microvessels in Prostate Cancer. Its Relevance for the Anti-angiogenetic Therapy
Current Cancer Therapy Reviews A Review on Novel Ligand Targeted Delivery for Cardiovascular Disorder
Current Drug Delivery Discovery of New Inhibitors of Urease Enzyme: A Study Using STD-NMR Spectroscopy
Letters in Drug Design & Discovery Sol-Gel Chemistry in Biosensing Devices of Electrical Transduction: Application to CEA Cancer Biomarker
Current Topics in Medicinal Chemistry Aza Analogs of Flavones as Potential Antimicrobial Agents
Letters in Drug Design & Discovery Synthesis of 4-piperidone Based Curcuminoids with Anti-inflammatory and Anti-Proliferation Potential in Human Cancer Cell Lines
Anti-Cancer Agents in Medicinal Chemistry Application of Solvent Influenced Fluorescence-quenching and Enhancement to Develop a Highly Sensitive HPLC Methodology for Analysis of Resveratrol-PEG Conjugates
Current Pharmaceutical Analysis