Abstract
In this study, we developed poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles with variable molecular PEG weights (2, 10, or 20 kDa) to encapsulate antifungal amphotericin B (AmB) and to evaluate its in vitro efficacy in strains of Candida sp. and in vitro cytotoxicity in human erythrocytes. The nanoparticles were prepared using an emulsification/solvent evaporation technique and were characterized with respect to size, size distribution, AmB entrapment efficiency, AmB state of aggregation, and AmB in vitro release profile. The mean particle size was 241 nm, and AmB encapsulation efficiency was above 68.9%. The AmB in vitro release profile demonstrated a burst effect within the first 24 h, which released approximately 20% of AmB, followed by a sustained release of approximately 29.5% of AmB over 26 days. The nanoparticles protected the erythrocytes from lysis caused by AmB within the first 12 h, and AmB was efficacious against strains of Candida sp. in its sustained release profile. The PEG molecular weight did not demonstrate an effect on the physicochemical and biological parameters. Thus, PLA-PEG blend nanoparticles may function as potential carriers for AmB.
Keywords: Amphotericin B, drug delivery, hemolysis, in vitro antifungal, polymeric nanoparticles.
Current Nanoscience
Title:Amphotericin B-Loaded Poly(Lactide)-Poly(Ethylene Glycol)-Blend Nanoparticles: Characterization and In Vitro Efficacy and Toxicity
Volume: 9 Issue: 5
Author(s): Caroline Danziato Rodrigues, Diani Meza Casa, Luciana Facco Dalmolin, Luciana Erzinger Alves de Camargo, Najeh Maissar Khalil and Rubiana Mara Mainardes
Affiliation:
Keywords: Amphotericin B, drug delivery, hemolysis, in vitro antifungal, polymeric nanoparticles.
Abstract: In this study, we developed poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles with variable molecular PEG weights (2, 10, or 20 kDa) to encapsulate antifungal amphotericin B (AmB) and to evaluate its in vitro efficacy in strains of Candida sp. and in vitro cytotoxicity in human erythrocytes. The nanoparticles were prepared using an emulsification/solvent evaporation technique and were characterized with respect to size, size distribution, AmB entrapment efficiency, AmB state of aggregation, and AmB in vitro release profile. The mean particle size was 241 nm, and AmB encapsulation efficiency was above 68.9%. The AmB in vitro release profile demonstrated a burst effect within the first 24 h, which released approximately 20% of AmB, followed by a sustained release of approximately 29.5% of AmB over 26 days. The nanoparticles protected the erythrocytes from lysis caused by AmB within the first 12 h, and AmB was efficacious against strains of Candida sp. in its sustained release profile. The PEG molecular weight did not demonstrate an effect on the physicochemical and biological parameters. Thus, PLA-PEG blend nanoparticles may function as potential carriers for AmB.
Export Options
About this article
Cite this article as:
Rodrigues Danziato Caroline, Casa Meza Diani, Dalmolin Facco Luciana, Camargo Erzinger Alves de Luciana, Khalil Maissar Najeh and Mainardes Mara Rubiana, Amphotericin B-Loaded Poly(Lactide)-Poly(Ethylene Glycol)-Blend Nanoparticles: Characterization and In Vitro Efficacy and Toxicity, Current Nanoscience 2013; 9 (5) . https://dx.doi.org/10.2174/15734137113099990072
DOI https://dx.doi.org/10.2174/15734137113099990072 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Central Nervous System Acting Drugs in Treatment of Migraine Headache
Central Nervous System Agents in Medicinal Chemistry Classic β-Amyloid Deposits Cluster Around Large Diameter Blood Vessels Rather than Capillaries in Sporadic Alzheimers Disease
Current Neurovascular Research Novelty in Inflammation and Immunomodulation in Migraine
Current Pharmaceutical Design Cell-Penetrating Peptide-Mediated Therapeutic Molecule Delivery into the Central Nervous System
Current Neuropharmacology Systemic Fungal Infections Caused by Candida Species: Epidemiology, Infection Process and Virulence Attributes
Current Drug Targets Postoperative Care of the Transplanted Patient
Current Cardiology Reviews A Dig Deep to Scout the Pharmacological and Clinical Facet of Garlic (<i>Allium sativum</i>)
Current Traditional Medicine Targeting to 5-HT1F Receptor Subtype for Migraine Treatment: Lessons from the Past, Implications for the Future
Central Nervous System Agents in Medicinal Chemistry Spatial Correlations between the Vacuolation, Prion Protein (PrPsc) Deposits and the Cerebral Blood Vessels in Sporadic Creutzfeldt-Jakob Disease
Current Neurovascular Research Arboviral Encephalitis and RNAi Treatment
Central Nervous System Agents in Medicinal Chemistry Nitrergic Response to Clostridium perfringens Infection in the Rat Brain Regions Effect of Red Light Irradiation
Central Nervous System Agents in Medicinal Chemistry Immune Responses to Adenovirus and Adeno-Associated Vectors Used for Gene Therapy of Brain Diseases: The Role of Immunological Synapses in Understanding the Cell Biology of Neuroimmune Interactions
Current Gene Therapy New Strategies and Patent Therapeutics in EBV-Associated Diseases
Mini-Reviews in Medicinal Chemistry Communicating Vaccine Safety to the Media and General Public
Current Drug Safety Fourth Generation of Synthetic Cannabinoid Receptor Agonists: A Review on the Latest Insights
Current Pharmaceutical Design Effect of Immunoglobulin Therapy on Blood Viscosity and potential concerns of Thromboembolism, Especially in Patients with Acute Kawasaki Disease
Recent Patents on Cardiovascular Drug Discovery Myalgic Encephalomyelitis: Symptoms and Biomarkers
Current Neuropharmacology Emerging Treatments in Acute Lymphoblastic Leukemia
Current Cancer Drug Targets Recent Patents Concerning Modulators of Protein Kinase C
Recent Patents on DNA & Gene Sequences Antiepileptic Drug-Induced Hypersensitivity Syndrome Reactions
Current Drug Safety