Abstract
In soybean, a small hormone like peptide, leginsulin was found to bind Basic 7S Globulin (Bg7S) and stimulate its tyrosine kinase activity. The NMR-structure of leginsulin, along with crystal structure of Bg7S, was used to create a rigid docking model, followed by flexible refinement of the interaction complex. This study provides structural insights into the binding of leginsulin to Bg7S. The complex is stabilized predominantly by hydrophobic forces and hydrogen bonds. The cleft is lined to a large extent by the cysteine rich region of the α-subunit of chain D as well as the carboxylterminal region of α-subunit of chain A. The model is in agreement with the available experimental evidence for the hotspot regions of Bg7S and leginsulin. We also observed another similar site on the surface of Bg7S by virtue of its pseudo- 222 symmetry. We have further hypothesized that two leginsulin molecules may bind to Bg7S in its crystal structure or tetrameric form.
Keywords: Soybean, Leginsulin, Basic 7S Globulin, 4kDa peptide, Protein-peptide docking, Tyrosine kinase activity.
Letters in Drug Design & Discovery
Title:Interaction between Basic 7S Globulin and Leginsulin in Soybean [Glycine max]: A Structural Insight
Volume: 11 Issue: 2
Author(s): Amandeep Singh, Prasoon Kumar Thakur, Megha Meena, Dhiraj Kumar, Sonika Bhatnagar, Ashok K. Dubey and Md. Imtaiyaz Hassan
Affiliation:
Keywords: Soybean, Leginsulin, Basic 7S Globulin, 4kDa peptide, Protein-peptide docking, Tyrosine kinase activity.
Abstract: In soybean, a small hormone like peptide, leginsulin was found to bind Basic 7S Globulin (Bg7S) and stimulate its tyrosine kinase activity. The NMR-structure of leginsulin, along with crystal structure of Bg7S, was used to create a rigid docking model, followed by flexible refinement of the interaction complex. This study provides structural insights into the binding of leginsulin to Bg7S. The complex is stabilized predominantly by hydrophobic forces and hydrogen bonds. The cleft is lined to a large extent by the cysteine rich region of the α-subunit of chain D as well as the carboxylterminal region of α-subunit of chain A. The model is in agreement with the available experimental evidence for the hotspot regions of Bg7S and leginsulin. We also observed another similar site on the surface of Bg7S by virtue of its pseudo- 222 symmetry. We have further hypothesized that two leginsulin molecules may bind to Bg7S in its crystal structure or tetrameric form.
Export Options
About this article
Cite this article as:
Singh Amandeep, Kumar Thakur Prasoon, Meena Megha, Kumar Dhiraj, Bhatnagar Sonika, K. Dubey Ashok and Imtaiyaz Hassan Md., Interaction between Basic 7S Globulin and Leginsulin in Soybean [Glycine max]: A Structural Insight, Letters in Drug Design & Discovery 2014; 11 (2) . https://dx.doi.org/10.2174/15701808113109990060
DOI https://dx.doi.org/10.2174/15701808113109990060 |
Print ISSN 1570-1808 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-628X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Dual-target Inhibitors Based on BRD4: Novel Therapeutic Approaches for Cancer
Current Medicinal Chemistry Gold Nanoparticles - Synthesis and Applications in Cancer Management
Recent Patents on Materials Science Identification of Inhibitors Based on Molecular Docking: Thyroid Hormone Transmembrane Transporter MCT8 as a Target
Current Drug Discovery Technologies MYC-Mediated Synthetic Lethality for Treating Tumors
Current Cancer Drug Targets PIM1 Kinase as a Target in Prostate Cancer: Roles in Tumorigenesis, Castration Resistance, and Docetaxel Resistance
Current Cancer Drug Targets Novel Approaches in the Treatment of Antipsychotic-Induced Hyperprolactinemia: The Role of Partial Agonists of D2 Dopaminergic Receptors
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Biomedical Applications of Protein Microarrays
Current Medicinal Chemistry Breast Cancer and Flavonoids - A Role in Prevention
Current Pharmaceutical Design Mannosylated Solid Lipid Nanocarriers of Chrysin to Target Gastric Cancer: Optimization and Cell Line Study
Current Drug Delivery A Review on Mechanisms of Anti Tumor Activity of Chalcones
Anti-Cancer Agents in Medicinal Chemistry Oxidative and Nitrosative Stress and Immune-inflammatory Pathways in Patients with Myalgic Encephalomyelitis (ME)/Chronic Fatigue Syndrome (CFS)
Current Neuropharmacology Image Guided Biopsy of Musculoskeletal Lesions with Low Diagnostic Yield
Current Medical Imaging The Therapeutic Potential of Melatonin in Neurological Disorders
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Cytotoxic Effects of the Monomer and Dimer of 3, 4-seco-ent-kaurene from Croton Micans and their Interaction with Antitumoral Drugs on Cellular Line of Human Prostate Cancer
Letters in Drug Design & Discovery Antiangiogenic Therapy
Current Pharmaceutical Design Garcinia Benzophenones Inhibit the Growth of Human Colon Cancer Cells and Synergize with Sulindac Sulfide and Turmeric
Anti-Cancer Agents in Medicinal Chemistry Technologies for Translational Imaging Using Generators in Oncology
Recent Patents on Anti-Cancer Drug Discovery Recent Therapeutic Advances for Treating Medulloblastoma: Focus on New Molecular Targets
CNS & Neurological Disorders - Drug Targets Base Excision Repair, the Redox Environment and Therapeutic Implications
Current Molecular Pharmacology Assays for Identification of Hsp90 Inhibitors and Biochemical Methods for Discriminating their Mechanism of Action
Current Topics in Medicinal Chemistry