Abstract
Carotenoids are considered to act as antioxidants and protect humans from serious disorders such as skin degeneration and ageing, cardiovascular disease, certain types of cancer, and age-related diseases of the eye. In this study, these chemopreventive activities of a carotenoids-overexpressing transgenic carrot were evaluated. The results of DPPH, hydroxyl, and superoxide radical scavenging tests demonstrate that the acetone extract obtained from the taproots of the carrot plants exhibits significant antioxidant activity. A higher activity was detected in the transgenic carrot extract compared with the wild-type extract. A chemopreventive activity test for degenerative diseases of the eye revealed that pretreatment with the carrot extract reduced cell death in a retinal ganglion cell line, RGC-5 cells exposed to 1-buthionine- (R,S)-sulfoximine and L-glutamic acid.
Keywords: Carotenoids, transgenic carrot, antioxidant activity, RGC-5 cells.
Current Drug Targets
Title:Enhanced Antioxidant and Protective Activities on Retinal Ganglion Cells of Carotenoids-Overexpressing Transgenic Carrot
Volume: 14 Issue: 9
Author(s): Kee Dong Yoon, Suk-Nam Kang, Ji-Yeong Bae, Haeng-Soon Lee, Sang-Soo Kwak, Insurk Jang, Il-Suk Kim, Cheol Ho Lee, Jung Myung Bae, Shin Woo Lee and Mi-Jeong Ahn
Affiliation:
Keywords: Carotenoids, transgenic carrot, antioxidant activity, RGC-5 cells.
Abstract: Carotenoids are considered to act as antioxidants and protect humans from serious disorders such as skin degeneration and ageing, cardiovascular disease, certain types of cancer, and age-related diseases of the eye. In this study, these chemopreventive activities of a carotenoids-overexpressing transgenic carrot were evaluated. The results of DPPH, hydroxyl, and superoxide radical scavenging tests demonstrate that the acetone extract obtained from the taproots of the carrot plants exhibits significant antioxidant activity. A higher activity was detected in the transgenic carrot extract compared with the wild-type extract. A chemopreventive activity test for degenerative diseases of the eye revealed that pretreatment with the carrot extract reduced cell death in a retinal ganglion cell line, RGC-5 cells exposed to 1-buthionine- (R,S)-sulfoximine and L-glutamic acid.
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Yoon Dong Kee, Kang Suk-Nam, Bae Ji-Yeong, Lee Haeng-Soon, Kwak Sang-Soo, Jang Insurk, Kim Il-Suk, Lee Ho Cheol, Bae Myung Jung, Lee Woo Shin and Ahn Mi-Jeong, Enhanced Antioxidant and Protective Activities on Retinal Ganglion Cells of Carotenoids-Overexpressing Transgenic Carrot, Current Drug Targets 2013; 14 (9) . https://dx.doi.org/10.2174/1389450111314090008
DOI https://dx.doi.org/10.2174/1389450111314090008 |
Print ISSN 1389-4501 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-5592 |
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New drug therapy for eye diseases
Eyesight is one of the most critical senses, accounting for over 80% of our perceptions. Our quality of life might be significantly affected by eye disease, including glaucoma, diabetic retinopathy, dry eye, etc. Although the development of microinvasive ocular surgery reduces surgical complications and improves overall outcomes, medication therapy is ...read more
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