Abstract
Polyols or sugar alcohols are sugar-derived metabolites. The origin, metabolic fate and functions of most of them are not clear. The monitoring of polyols can be helpful in the diagnosis and treatment of diseases, especially those of unknown etiology. The main aim of the study was to develop and demonstrate the efficiency, reliability, sensitivity of the gas chromatography - electron capture detection method for the quantification of polyols: D-arabitol, ribitol and erythritol in urine. In the case of polyols there is a problem with the preparation of samples for analysis. Enrichment and separation of analytes and extract purification are difficult because of their relatively high polarity. That is why we have developed the method of sample preparation based on solid phase extraction with using non-porous graphitized carbon. The elaborated method was applied in autistic children in relation to the pentose phosphate pathway metabolism.
Keywords: Electron capture detection, Gas chromatography, Graphitized carbon black, Polyols, Solid phase extraction, Urine.
Current Organic Chemistry
Title:Gas Chromatography-electron Capture Detection Method for Determination of Polyols in the Urine
Volume: 17 Issue: 20
Author(s): Wioletta Grys, Joanna Kaluzna-Czaplinska and Jacek Rynkowski
Affiliation:
Keywords: Electron capture detection, Gas chromatography, Graphitized carbon black, Polyols, Solid phase extraction, Urine.
Abstract: Polyols or sugar alcohols are sugar-derived metabolites. The origin, metabolic fate and functions of most of them are not clear. The monitoring of polyols can be helpful in the diagnosis and treatment of diseases, especially those of unknown etiology. The main aim of the study was to develop and demonstrate the efficiency, reliability, sensitivity of the gas chromatography - electron capture detection method for the quantification of polyols: D-arabitol, ribitol and erythritol in urine. In the case of polyols there is a problem with the preparation of samples for analysis. Enrichment and separation of analytes and extract purification are difficult because of their relatively high polarity. That is why we have developed the method of sample preparation based on solid phase extraction with using non-porous graphitized carbon. The elaborated method was applied in autistic children in relation to the pentose phosphate pathway metabolism.
Export Options
About this article
Cite this article as:
Grys Wioletta, Kaluzna-Czaplinska Joanna and Rynkowski Jacek, Gas Chromatography-electron Capture Detection Method for Determination of Polyols in the Urine, Current Organic Chemistry 2013; 17 (20) . https://dx.doi.org/10.2174/13852728113179990031
DOI https://dx.doi.org/10.2174/13852728113179990031 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
Call for Papers in Thematic Issues
Advances of Heterocyclic Chemistry with Pesticide Activity
Global food safety and security will continue to be a global concern for the next 50 years and beyond. Plant diseases have had a significant impact on food safety and security throughout the entire food chain, from primary production to consumption. While conventional chemical pesticides have been traditionally used for ...read more
Calculation design of covalent/metal organic framework based catalysts
This research area combines theoretical computation and screening with machine learning for the design of covalent/metal organic framework-based catalysts, bridging the disciplines of organic chemistry, physical chemistry, computational chemistry, materials science, and machine learning. It covers several critical aspects: designing and synthesizing organic catalysts for improved performance, applying computational methods ...read more
Carbohydrates conversion in biofuels and bioproducts
Biomass pretreatment, hydrolysis, and saccharification of carbohydrates, and sugars bioconversion in biofuels and bioproducts within a biorefinery framework. Carbohydrates derived from woody biomass, agricultural wastes, algae, sewage sludge, or any other lignocellulosic feedstock are included in this issue. Simulation, techno-economic analysis, and life cycle analysis of a biorefinery process are ...read more
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Disease Progression in Children with Vertically-Acquired HIV Infection in Sub-Saharan Africa: Reviewing the Need for HIV Treatment
Current HIV Research Studies on Chloride Channels and their Modulators
Current Topics in Medicinal Chemistry Novel Targets in Multiple Sclerosis: To Oxidative Stress and Beyond
Current Topics in Medicinal Chemistry LFA-1 on Leukemic Cells as a Target for Therapy or Drug Delivery
Current Pharmaceutical Design Organophosphorus Chemistry: Therapeutic Intervention in Mechanisms of Viral and Cellular Replication
Current Organic Chemistry Sepsis-induced Cardiomyopathy
Current Cardiology Reviews Treatment of Leukoaraiosis: A Futuristic View
Current Drug Targets Emerging Treatment Strategies and Potential Therapeutic Targets in Primary Sjogrens Syndrome
Inflammation & Allergy - Drug Targets (Discontinued) Current Opinion in the Pharmaceutical Management of Irritable and Inflammatory Bowel Diseases: Role of ATP
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Rheumatoid Arthritis: An Evolutionary Force in Biologics
Current Pharmaceutical Design Gene Therapy Approaches in an Autoimmune Demyelinating Disease: Multiple Sclerosis
Current Gene Therapy PDE4D Gene in the STRK1 Region on 5q12: Susceptibility Gene for Ischemic Stroke
Current Medicinal Chemistry Phosphonomethoxyalkyl Analogs of Nucleotides
Current Pharmaceutical Design HIV-Infected Patients and Liver Transplantation: Who, When and Why
Current HIV Research An Update of the Published Reports on Biologics Use for Psoriasis and the Reimbursement Status in Asia-Pacific Region
Current Rheumatology Reviews Update on Myositis Therapy: From Today’s Standards to Tomorrow’s Possibilities
Current Pharmaceutical Design Posterior Reversible Encephalopathy Syndrome with Atypical Presentation: A Pictorial Review on MR Imaging Features
Current Medical Imaging Non-Celiac Gluten Sensitivity Triggers Gut Dysbiosis, Neuroinflammation, Gut-Brain Axis Dysfunction, and Vulnerability for Dementia
CNS & Neurological Disorders - Drug Targets Animal Models in Neurology: Drawbacks and Opportunities
Current Pharmaceutical Design Nonstandard Drugs and Feasible New Interventions for Autoimmune Hepatitis: Part II
Inflammation & Allergy - Drug Targets (Discontinued)