Abstract
Gout is a painful disease of joints generated because of the deposition of urate crystals within the joints. Uricase is an enzyme capable of degrading less water soluble urate crystals into a compound allantoin, which is more water soluble and hence is easily excreted. Uricase being proteinaceous is degraded by the protein digestive enzymes of gastro intestinal tract (GIT) when administered orally. Colonic part of GIT lacks protein digestive enzymes and thus provides suitable conditions for the absorption of uricase. Colon consists of several microbial flora which secrete enzymes capable of degradation of alginates and pectin which are not digested by upper GI tract. Eudragit S-100 (pH sensitive polymer) coated alginate – pectinate microbeads were found to be effective for delivery of uricase directly to the colonic site. Sodium taurocholate, a bile salt, was found to be suitable for the effective absorption of uricase from colonic site into the systemic circulation.
Keywords: Colon targeting, Enzyme, Enzyme therapy, Gout, Hyperuricemia, Microbeads, Uricase.
Drug Delivery Letters
Title:Fabrication and Evaluation of Colon Targeted Uricase Containing Microbeads for Treatment of Gout
Volume: 3 Issue: 1
Author(s): Harinath Dwivedi, Kusum Agarwal and Shubhini A Saraf
Affiliation:
Keywords: Colon targeting, Enzyme, Enzyme therapy, Gout, Hyperuricemia, Microbeads, Uricase.
Abstract: Gout is a painful disease of joints generated because of the deposition of urate crystals within the joints. Uricase is an enzyme capable of degrading less water soluble urate crystals into a compound allantoin, which is more water soluble and hence is easily excreted. Uricase being proteinaceous is degraded by the protein digestive enzymes of gastro intestinal tract (GIT) when administered orally. Colonic part of GIT lacks protein digestive enzymes and thus provides suitable conditions for the absorption of uricase. Colon consists of several microbial flora which secrete enzymes capable of degradation of alginates and pectin which are not digested by upper GI tract. Eudragit S-100 (pH sensitive polymer) coated alginate – pectinate microbeads were found to be effective for delivery of uricase directly to the colonic site. Sodium taurocholate, a bile salt, was found to be suitable for the effective absorption of uricase from colonic site into the systemic circulation.
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Dwivedi Harinath, Agarwal Kusum and Saraf Shubhini A, Fabrication and Evaluation of Colon Targeted Uricase Containing Microbeads for Treatment of Gout, Drug Delivery Letters 2013; 3 (1) . https://dx.doi.org/10.2174/2210304x11303010007
DOI https://dx.doi.org/10.2174/2210304x11303010007 |
Print ISSN 2210-3031 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-304X |
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