Abstract
The generation of genetically altered mice for research is invaluable for investigations into the underlying mechanisms of common complex diseases such as cancer as well as for therapeutic studies. The correct identification of the genotype of each mouse is therefore essential. Genotyping is routinely performed by using blood, tail or ear tissue; however, this can cause pain or discomfort to the animal and nor is amenable to scale up for high-throughput sampling and analyses. Genotyping from saliva, hair and rectal cells is less invasive, but still requires the restraint of the animal. In contrast, collection of stool samples for DNA isolation is non-invasive as well as repeatable and time efficient, with recent studies showing it to be as accurate as the alternative methods. Fecal DNA genotyping therefore provides an attractive technique for non-invasive genotyping. This feature article examines and synthesizes the recent advances in fecal genotyping with a view to mouse models for nutrigenomics cancer chemoprevention studies specifically, and personalized medicine more broadly.
Keywords: Cancer, feces, genotyping, non-invasive, novel diagnostic platforms, personalized medicine, transgenic mice
Current Pharmacogenomics and Personalized Medicine
Title:Fecal DNA Genotyping: A Non-invasive Approach to Characterize Mouse Models for Nutrigenomics Cancer Chemoprevention Studies
Volume: 11 Issue: 1
Author(s): Erin L. Symonds
Affiliation:
Keywords: Cancer, feces, genotyping, non-invasive, novel diagnostic platforms, personalized medicine, transgenic mice
Abstract: The generation of genetically altered mice for research is invaluable for investigations into the underlying mechanisms of common complex diseases such as cancer as well as for therapeutic studies. The correct identification of the genotype of each mouse is therefore essential. Genotyping is routinely performed by using blood, tail or ear tissue; however, this can cause pain or discomfort to the animal and nor is amenable to scale up for high-throughput sampling and analyses. Genotyping from saliva, hair and rectal cells is less invasive, but still requires the restraint of the animal. In contrast, collection of stool samples for DNA isolation is non-invasive as well as repeatable and time efficient, with recent studies showing it to be as accurate as the alternative methods. Fecal DNA genotyping therefore provides an attractive technique for non-invasive genotyping. This feature article examines and synthesizes the recent advances in fecal genotyping with a view to mouse models for nutrigenomics cancer chemoprevention studies specifically, and personalized medicine more broadly.
Export Options
About this article
Cite this article as:
L. Symonds Erin, Fecal DNA Genotyping: A Non-invasive Approach to Characterize Mouse Models for Nutrigenomics Cancer Chemoprevention Studies, Current Pharmacogenomics and Personalized Medicine 2013; 11 (1) . https://dx.doi.org/10.2174/1875692111311010004
DOI https://dx.doi.org/10.2174/1875692111311010004 |
Print ISSN 1875-6921 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6913 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Aceclofenac: Species-Dependent Metabolism and Newer Paradigm Shift from Oral to Non-oral Delivery
Current Topics in Medicinal Chemistry Vitamins B1, B2, B3 and B9 – Occurrence, Biosynthesis Pathways and Functions in Human Nutrition
Mini-Reviews in Medicinal Chemistry Contextualizing the Genes Altered in Bladder Neoplasms in Pediatric and Teen Patients Allows Identifying Two Main Classes of Biological Processes Involved and New Potential Therapeutic Targets
Current Genomics Use Of External Intrathecal Infusion Pumps In The Management Of Septic Complications: A Case Report
Endocrine, Metabolic & Immune Disorders - Drug Targets DJ-1: A New Comer in Parkinsons Disease Pathology
Current Molecular Medicine Mitochondria as a Therapeutic Target for Aging and Neurodegenerative Diseases
Current Alzheimer Research Vascular Calcification Revisited: A New Perspective for Phosphate Transport
Current Cardiology Reviews Genetic Aspects of Lone Atrial Fibrillation: What Do We Know?
Current Pharmaceutical Design Human Buccal Mucosa as an Innovative Site of Drug Delivery
Current Pharmaceutical Design Continuing Accommodation and Support Needs of Long-Term Patients with Severe Mental Illness in the Era of Community Care
Current Psychiatry Reviews Is There a Role for Cytokine Based Therapies in Fibromyalgia
Current Pharmaceutical Design The Role of the Cytoskeleton in the Life Cycle of Viruses and Intracellular Bacteria: Tracks, Motors, and Polymerization Machines
Current Drug Targets - Infectious Disorders Protein/ Hormone Based Nanoparticles as Carriers for Drugs Targeting Protein-Protein Interactions
Current Topics in Medicinal Chemistry Melanin Concentrating Hormone Receptor Antagonists as Antiobesity Agents: From M2 to MCHR-1
Current Topics in Medicinal Chemistry Do mtDNA Mutations Participate in the Pathogenesis of Sporadic Parkinsons Disease?
Current Genomics Intracerebral Hemorrhage and Diabetes Mellitus: Blood-Brain Barrier Disruption, Pathophysiology and Cognitive Impairments
CNS & Neurological Disorders - Drug Targets HCN Channels Modulators: The Need for Selectivity
Current Topics in Medicinal Chemistry Schizophrenia and the Neglect Syndrome: Parietal Contributions to Cognitive Dysfunction in Schizophrenia
Current Psychiatry Reviews Microglial Activation and its Implications in the Brain Diseases
Current Medicinal Chemistry Chondroitin Sulphate for the Treatment of Osteoarthritis
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents