Abstract
Many compounds can interact with DNA leading to changes of DNA structure as point mutation and bases excision, which could trigger some metabolic failures, which leads to the changes in DNA structure resulting in cancer. Oxidation of nucleic acid bases belongs to the one of the mostly occurred type of DNA damaging leading to the above mentioned phenomena. The investigation of processes of DNA oxidation damage is topical and electrochemical methods include a versatile and sensitive tool for these purposes. 8-hydroxydeoxyguanosine (8-OHdG) is the most widely accepted marker of DNA damage. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generate several other products within the double helix besides mentioned oxidation products of nucleic acid bases. The basic electrochemical behaviour of nucleic acids bases on various types of carbon electrodes is reviewed. Further, we address our attention on description of oxidation mechanisms and on detection of the most important products of nucleic bases oxidation. The miniaturization of detector coupled with some microfluidic devices is suggested and discussed.
The main aim of this review is to report the advantages and features of the electrochemical detection of guanine oxidation product as 8-OHdG and other similarly produced molecules as markers for DNA damage.
Keywords: Miniaturization, nucleic acid bases, oxidation damage of DNA, voltammetry.
Combinatorial Chemistry & High Throughput Screening
Title:Electrochemical Study of DNA Damaged by Oxidation Stress
Volume: 16 Issue: 2
Author(s): Ondrej Zitka, Sona Krizkova, Sylvie Skalickova, Pavel Kopel, Petr Babula, Vojtech Adam and Rene Kizek
Affiliation:
Keywords: Miniaturization, nucleic acid bases, oxidation damage of DNA, voltammetry.
Abstract: Many compounds can interact with DNA leading to changes of DNA structure as point mutation and bases excision, which could trigger some metabolic failures, which leads to the changes in DNA structure resulting in cancer. Oxidation of nucleic acid bases belongs to the one of the mostly occurred type of DNA damaging leading to the above mentioned phenomena. The investigation of processes of DNA oxidation damage is topical and electrochemical methods include a versatile and sensitive tool for these purposes. 8-hydroxydeoxyguanosine (8-OHdG) is the most widely accepted marker of DNA damage. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generate several other products within the double helix besides mentioned oxidation products of nucleic acid bases. The basic electrochemical behaviour of nucleic acids bases on various types of carbon electrodes is reviewed. Further, we address our attention on description of oxidation mechanisms and on detection of the most important products of nucleic bases oxidation. The miniaturization of detector coupled with some microfluidic devices is suggested and discussed.
The main aim of this review is to report the advantages and features of the electrochemical detection of guanine oxidation product as 8-OHdG and other similarly produced molecules as markers for DNA damage.
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Cite this article as:
Zitka Ondrej, Krizkova Sona, Skalickova Sylvie, Kopel Pavel, Babula Petr, Adam Vojtech and Kizek Rene, Electrochemical Study of DNA Damaged by Oxidation Stress, Combinatorial Chemistry & High Throughput Screening 2013; 16 (2) . https://dx.doi.org/10.2174/1386207311316020007
DOI https://dx.doi.org/10.2174/1386207311316020007 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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