Abstract
Shigella flexneri is a Gram-negative bacillus that is responsible for a severe form of dysentery called Shigellosis, which mainly affects children and the elderly in both underdeveloped and developed countries. Pathogenic S. flexneri strains possess a large virulence plasmid that codes for effector proteins that are required for the entry and spread of the bacteria into colonocytes. Among these proteins is the translocator IpaC, which plays an important role in the invasion process; IpaC is implicated in pore formation in the host cell membrane and induces cytoskeletal rearrangements in macrophages and epithelial cells, thereby promoting bacterial entry. The ability of IpaC to insert into the plasma membrane is due to a large nonpolar region of the protein structure. This characteristic also renders difficulties in recovery and purification when the protein is expressed in E. coli. Several works have considered different methodologies for the improved production and purification of IpaC. Herein, we propose an alternative method that is based on changes in the induction temperature and extraction buffer to facilitate the accumulation of high yields of soluble proteins for their further processing and ultimate use in biotechnological approaches.
Keywords: Shigella flexneri, IpaC production, purification, shigellosis, vaccines
Protein & Peptide Letters
Title:Cloning and Purification of IpaC Antigen from Shigella flexneri: Proposal of a New Methodology
Volume: 20 Issue: 2
Author(s): Cristiane Mobilon, Marcelo Augusto Szymanski de Toledo, Fernanda Laroza Paganelli, Clelton Aparecido dos Santos, Fernanda de Pace, Jacqueline Boldrin de Paiva, Eliana Guedes Stehling, Gerson Nakazato, Aline Gambaro Balieiro, Flavia Pereira da Silva Airoldi, Francisco de Assis Machado Reis and Wanderley Dias da Silveira
Affiliation:
Keywords: Shigella flexneri, IpaC production, purification, shigellosis, vaccines
Abstract: Shigella flexneri is a Gram-negative bacillus that is responsible for a severe form of dysentery called Shigellosis, which mainly affects children and the elderly in both underdeveloped and developed countries. Pathogenic S. flexneri strains possess a large virulence plasmid that codes for effector proteins that are required for the entry and spread of the bacteria into colonocytes. Among these proteins is the translocator IpaC, which plays an important role in the invasion process; IpaC is implicated in pore formation in the host cell membrane and induces cytoskeletal rearrangements in macrophages and epithelial cells, thereby promoting bacterial entry. The ability of IpaC to insert into the plasma membrane is due to a large nonpolar region of the protein structure. This characteristic also renders difficulties in recovery and purification when the protein is expressed in E. coli. Several works have considered different methodologies for the improved production and purification of IpaC. Herein, we propose an alternative method that is based on changes in the induction temperature and extraction buffer to facilitate the accumulation of high yields of soluble proteins for their further processing and ultimate use in biotechnological approaches.
Export Options
About this article
Cite this article as:
Mobilon Cristiane, Augusto Szymanski de Toledo Marcelo, Laroza Paganelli Fernanda, Aparecido dos Santos Clelton, de Pace Fernanda, Boldrin de Paiva Jacqueline, Guedes Stehling Eliana, Nakazato Gerson, Gambaro Balieiro Aline, Pereira da Silva Airoldi Flavia, de Assis Machado Reis Francisco and Dias da Silveira Wanderley, Cloning and Purification of IpaC Antigen from Shigella flexneri: Proposal of a New Methodology, Protein & Peptide Letters 2013; 20 (2) . https://dx.doi.org/10.2174/0929866511320020003
DOI https://dx.doi.org/10.2174/0929866511320020003 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Extranuclear Localization of SIRT1 and PGC-1α: An Insight into Possible Roles in Diseases Associated with Mitochondrial Dysfunction
Current Molecular Medicine Albumin Infusion Therapy in Stroke, Sepsis and the Critically Ill
Current Nutrition & Food Science Mechanisms And Prevention Of TAVI-Related Cerebrovascular Events
Current Pharmaceutical Design Atherogenesis in White Coat Hypertension
Current Hypertension Reviews Transcatheter Aortic Valve Technology: Current State
Recent Patents on Biomedical Engineering (Discontinued) Role of Azoles in Cancer Prevention and Treatment: Present and Future Perspectives
Anti-Cancer Agents in Medicinal Chemistry Experiments on Analysing Voice Production: Excised (Human, Animal) and In Vivo (Animal) Approaches
Current Bioinformatics Preeclampsia: A Couples Disease with Maternal and Fetal Manifestations
Current Pharmaceutical Design In Silico Prediction of Drug Properties
Current Medicinal Chemistry Adenosine and ATP Receptors in the Brain
Current Topics in Medicinal Chemistry Uteroplacental Circulation and Fetal Vascular Function and Development
Current Vascular Pharmacology Physical Function and Exercise in Older Patients with Cardiovascular and Respiratory Conditions
Current Pharmaceutical Design Targeting the L-Arginine-Nitric Oxide Pathway for Cancer Treatment
Current Pharmaceutical Design Lipid Lowering Drugs and Gallstones: A Therapeutic Option?
Current Pharmaceutical Design Opioid-induced Cardioprotection
Current Pharmaceutical Design Role of α- and β-adrenergic Mechanisms in the Pathogenesis of Pulmonary Injuries Characterized by Edema, Inflammation and Fibrosis
Cardiovascular & Hematological Disorders-Drug Targets Synthetic and Medicinal Prospective of Structurally Modified Curcumins
Current Topics in Medicinal Chemistry Extracellular Matrix Remodeling in Hypertension
Current Hypertension Reviews Cardiovascular and Fluid Volume Control in Humans in Space
Current Pharmaceutical Biotechnology Compounds that Combine Aldose Reductase Inhibitory Activity and Ability to Prevent the Glycation (Glucation and/or Fructation) of Proteins as Putative Pharmacotherapeutic Agents
Drug Design Reviews - Online (Discontinued)