Preliminary Study of microRNAs Allele-Specific Targeting in Allergic Rhinitis Patients from Central China
Background: Abnormal expression of miRNA is a common feature in many diseases. Some studies have also emphasized that miRNAs play an important role in asthma and allergic rhinitis (AR). This study attempts to reveal the differences between miRNAs expression and normal nasal mucosa in AR patients by microarray method, so as to further understand the molecular mechanism of AR development.
Method: MiRNA microrrays were used for analyzing six samples of the nasal mucosa of AR and six samples of nonallergic patients. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) of some differentially expressed miRNAs was used to confirm the array results. Furthermore, pathway analysis was carried out.
Results: The microarray identified that 64 miRNAs exhibited altered expression in the nasal mucosa of the AR group when compared with the control group. Moreover, the expression levels of ten miRNAs were significantly altered in the AR group. To verify the results of the microarray, three differentially expressed miRNA were determined by RT-PCR, and the results also confirmed these changes. Ten differentially expressed miRNAs were present in the nasal mucosa of AR patients compared with the control group, and three differentially expressed miRNAs, as miR-1244, miR-4651 and miR-7641, were determined by RT-PCR, indicating that they play important roles in the process of AR.
Conclusion: miR-1244, miR-4651 and miR-7641 may play important roles in the process of AR. Sequencing analysis indicated that three kinds of mutations exist in MAPK8 3’UTR, which may play a role in binding with miR-7641, and then influence the AR process. Single miRNA or, more probably, their sets hold the promise for their use as biomarkers of allergic rhinitis. They may also be promising targets in future therapies.
Combinatorial Chemistry & High Throughput Screening