Recent advances in the fields of genomics and bioinformatics have made it increasingly clear that genetic sequence alone cannot explain how the genome regulates the development and function of complex multicellular organisms both in health and disease. This inference has led to the expansion of epigenetics as a discipline. Epigenetics refers to the way in which the environment in the wide sense participates in the regulation of gene expression. Several studies show that the well-known beneficial role of a healthy lifestyle over a number of pathologies or as a pre-emptive therapy is at least in part exerted through epigenetic mechanisms, thus giving rise to a new paradigm of preventive medicine based on the concept of genetic plasticity. In Epigenetics of Lifestyle, several contributors provide a comprehensive view of how various facets of lifestyle, including nutrition, exercise, stress, addiction or social interactions, affect chromatin (the combination of DNA and proteins that make up the contents of a cell nucleus) - resulting in profound and long-lasting changes in gene function. In summary, Epigenetics of Lifestyle is a fresh approach towards epigenetics and presents the reader with significant research findings in epigenetics and lifestyle studies. This volume is a simplified source of information for both undergraduate and working professionals interested in lifestyle medicine and life sciences in general.

RNA interference (RNAi) is a biological process in which small nucleotide sequences destroy specific mRNA molecules and, therefore, interrupt genetic expression. RNAi has rapidly developed into a widely used technique in biotechnology, experimental biology, genomics and medicine. Frontiers in RNAi is a collection of research articles covering the applications of RNAi principles and technologies and has been compiled by members of the RNAi Global Initiative, a collection of academic and industrial groups with extensive experience in genome-wide RNAi screening. Chapters included in the first volume touch on some of the recent advances in RNAi technology (i.e., applications to 3D cell culture, drug repurposing, and bioproduction) and provide guidance on how to address the continued challenges associated with off-target effects, database structuring, and pooled shRNA screens.

This reference is a timely compilation of studies of genome size and genetic stability of regenerated plants. It presents 13 book chapters that cover recent advancements in CRISPR/Cas-based genome editing, the use of molecular markers to analyze somaclonal variation in tissue culture, and genetic stability assessment in various plant species, including medicinally valuable plants like Valeriana and Coffea. The book also highlights the role of flow cytometry in investigating polyploidy and provides valuable insights into genetic fidelity assessment of micropropagated woody plants and orchids. The contributors have shed light on the intra-specific and inter-specific genome and chromosome number variation with reference to gene duplication and DNA sequence loss. Molecular techniques for detecting ploidy levels and genetic homogeneity in regenerated plantlets are also discussed. Additional highlights of the book include brief guidelines for experimental protocols for flow cytometry and molecular markers, coverage of a wide range of plants, and supporting references. This is an excellent reference for biologists, geneticists, and plant scientists exploring genetic homogeneity and genome size variation in diverse plant groups.

Introduction to Molecular Genomics introduces the college student to the fundamental concepts of molecular biology and genomics. The text puts an emphasis on important topics in the subject that contribute to the learner’s understanding. These topics include molecular genomics, biodiversity and molecular phenomenon behind evolution of species, modern molecular methods for enhanced genomics research, DNA modifications at the molecular level for transgenic animal species, the role of cell environment on the gene expression, to name a few. The book has been designed to suit the requirements of educational courses in molecular biology, genomics and biochemistry. <p> Key features <p> - Covers basic concepts on key topics in molecular biology and genomics <p> - Simple easy-to-read layout <p> - Includes references for further reading <p> - Includes a section on ethical aspects of scientific research <p> Introduction to Molecular Genomics is a simple primer for students in applied or advanced life science courses at undergraduate levels

Nucleic acid sequencing techniques have enabled researchers to determine the exact order of base pairs - and by extension, the information present - in the genome of living organisms. Consequently, our understanding of this information and its link to genetic expression at molecular and cellular levels has lead to rapid advances in biology, genetics, biotechnology and medicine. Next-Generation Sequencing and Sequence Data Analysis is a brief primer on DNA sequencing techniques and methods used to analyze sequence data. Readers will learn about recent concepts and methods in genomics such as sequence library preparation, cluster generation for PCR technologies, PED sequencing, genome assembly, exome sequencing, transcriptomics and more. This book serves as a textbook for students undertaking courses in bioinformatics and laboratory methods in applied biology. General readers interested in learning about DNA sequencing techniques may also benefit from the simple format of information presented in the book.

Genetic disorders have been the focus of scientists for a long time. The emergence of next-generation sequencing techniques has ushered a new era in genetics and several developments have occurred in human genetics. The scientific perspective has also been widened with omics technologies that allow researchers to analyze genetic sequences and their expression products. An integrated approach is being used not only for diagnosis but also for disease management and therapeutic purposes. This book highlights emerging areas of omics technology and its application in the diagnosis and management of human genetic disorders. The book covers three areas of research and implementation: - Diagnosis (covering conventional strategies to next-generation platforms). This section focuses on the role of in silico analysis, databases and multi-omics of single-cell which will help in designing better management strategies. - Disease Management and therapeutic interventions. This section starts with genetic counselling and progresses to more specific techniques such as pharmacogenomics and personalized medicine, gene editing techniques and their applications in gene therapies and regenerative medicine. - Case studies. This section discusses the applications and success of all the above-mentioned strategies on selected human disorders. This book serves as a handy reference for students and academics studying advanced omics techniques in biochemistry and molecular genetics as part of courses in life sciences, pharmacology and medicine.

This reference is a comprehensive review of genome editing in bacteria. The multi-part book meticulously consolidates research findings and insights on the applications of bacteria across several industries, including food processing and pharmaceutical development. The book covers four overarching themes for readers: a historical perspective of genome editing, genome editing in probiotics, applications of genome editing in agricultural microbiology and genetic engineering in environmental microbiology. The editors have also compiled chapters that provide an in-depth analysis of gene regulation and metabolic engineering through genome editing tools for specific bacteria. Key topics in part 2: - Targeting pathogenic microbes for plants and animals using CRISPR-CAS - Genome editing microbes to improve crop yield plant growth for sustainable agriculture - Applications of genome editing for bioremediation - Microbial genome editing for environmental bioprocessing - Genetic engineering for methanotrophs - Genome engineering in Cyanobacteria - Genome editing in Streptomyces Genome Editing in Bacteria is a definitive reference for scholars, researchers and industry professionals navigating the forefront of bacterial genomics.

This laboratory manual is designed to introduce beginner level researchers to the essential experimental techniques of molecular cloning. With a strong focus on hands-on protocols and a clear, cloning-centric framework, the book simplifies complex methods while building a strong foundation in molecular biology. Across eight structured chapters, the manual initially covers topics such as laboratory safety and fundamental skills, then progresses through microbiological techniques, DNA isolation and purification, DNA analysis, recombinant DNA construction to clone identification. The final chapter includes detailed appendices outlining standard reagent compositions and preparation methods. Special emphasis is placed on the rationale behind each procedure, making the learning process both practical and conceptually grounded. Key features: Explains experimental protocols with step-by-step clarity Gives rationale and mode of action behind each procedure Emphasizes critical steps through italicized notes and tips Provides special information panels for deeper contextual knowledge Include comprehensive appendices for reagent preparation and reference

Genetic Diversity of Coronaviruses (Volume 2) comprehensively analyzes the genetic evolution, mutations, and host interactions of three significant coronaviruses: SARS-CoV, MERS-CoV, and SARS-CoV-2. This book examines the historical development, molecular epidemiology, and genetic adaptations that have driven the emergence of new variants, shaping the trajectory of global outbreaks. Organized into four parts, the book begins with the genetic dynamics of SARS-CoV and MERS-CoV, followed by an in-depth exploration of SARS-CoV-2. It concludes with insights into how coronaviruses interact with host disorders, including epigenetics and neurological implications. Advanced topics, such as the role of artificial intelligence in understanding coronaviruses, are also discussed. Key Features: - Detailed examination of genetic mutations in SARS-CoV, MERS-CoV, and SARS-CoV-2. - Analysis of host genetic diversity and emerging variants. - Connections between coronaviruses and epigenetic or neurological disorders. - Applications of AI in coronavirus research.

Thriving Across the Lifespan and Around the Globe: Day in the Life Visual Research Approach is the result of several decades of international, pan-disciplinary research-team collaborations using quasi-ecological visual methodologies to investigate the psychosocial development of children in diverse communities. The initial studies highlighted in this work involved filming a ‘Day in the Life’ of toddlers and primary school children, their families, and schools in diverse communities in Asia, South and North America, and Europe. Filmed in their homes and communities for an entire day, the youngest participants were commonly seen to be thriving in diverse psychosocial domains, such as in their security strivings, emergent literacy and graphic representations, and musicality. Research shows that the nature of a child's development can vary in as many ways as there are diverse contexts. Thriving in the transition from home to primary school is a second developmental milestone investigated in the research. The book serves as an account of the lived experiences of thriving children,among their families, their schools and their communities. The contents give an invaluable insight into the psychology of early childhood while giving the reader an opportunity to understand the resilience of mobile early teenagers as well as independent older adults. The contributions in the book also provide an additional layer to our understanding of visual ethnography by covering such phenomena as agency and communitarianism, spirituality, and the place of humour in the context of challenges encountered across the lifespan. Through presenting quasi-ecological experimental approaches, this reference enhances the reader’s insight into the texture and nature of thriving in situ, in natural contexts.