Methods to Determine Enzymatic Activity

Assays of Peroxidase Activity

Author(s): Bernardo Dias Ribeiro and Maria Alice Zarur Coelho

Pp: 27-38 (12)

DOI: 10.2174/9781608053001113010006

* (Excluding Mailing and Handling)


Peroxidases are enzymes that use various peroxides as electron acceptors in oxidation, and are widespread in nature. Their activities are usually determined by spectrophotometric methods, which a condensation or oxidation product is formed in the presence of H2O2, or by titrimetric methods, using potassium permanganate or sodium thiosulfate, which measure free hydrogen peroxide in solution.

Keywords: Peroxidase, hydrogen peroxide, alkyl peroxides, benzyl peroxide, catalase, lactoperoxidase, myeloperoxidase, horseradish peroxidase, vanadium bromoperoxidase, lignin peroxidase, glutathione peroxidase, cytochrome C peroxidase, guaiacol.

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