Lipid homeostasis is essential for the maintenance of the organism energy balance while enabling the cells to perform vital functions. In mammals, improper control of these metabolic pathways can result in serious health problems, such as obesity, increased risk of coronary artery diseases, diabetes and related problems, such as hypertension and lipidemia. The nuclear receptor peroxisome proliferator-activated receptorγ (PPARγ), together with its heterodimeric partner retinoid X receptor (RXR), is a master regulator of adipocyte differentiation, being involved in the regulation of food intake, metabolic efficiency and energy storage. Triorganotins such as tributyltin (TBT) and triphenyltin (TPhT) are high affinity ligands of both RXRs and PPARγ. In line with these findings, the use of the 3T3-L1 cell system, a well characterized model for adipogenesis, demonstrated that organotins stimulate 3T3-L1 cells differentiation and the expression of adipocyte marker genes. In vivo exposure of neonate mice to TBT significantly elevates lipid accumulation in several tissues, thus supporting the hypothesis that chronic lifetime human exposure to organotins may increase the risk of obesity. While PPAR has not been described outside deuterostomes, RXR is ubiquitous within metazoans. Thus, the taxonomic scope for organotin-mediated lipid homeostasis disruption may be wider than initially anticipated. This is further supported by the observation of increased lipid accumulation in amphibians and teleost fish and changes to the lipid profile in molluscs after organotin exposure. Therefore, we review here the current evidence on the molecular and biochemical mechanisms for organotin-mediated lipid homeostasis disruption within several metazoan phyla.