Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach

Colorimetric Indicator Displacement Assays (CIDAs)

Author(s): Ishfaq Ahmad Rather* and Rashid Ali * .

Pp: 35-59 (25)

DOI: 10.2174/9789815165913124010004

* (Excluding Mailing and Handling)

Abstract

Owing to the unique advantages of naked eye color detection in the sensing of various important analytes, colorimetric indicator displacement assay (C-IDA) has received its recognition as the principal evolutionary extension of IDA. This chapter, besides dealing with all conceptual details of C-IDA, also offers basic criteria for the development and classification of chromogenic sensing ensembles. A plethora of examples in order to elucidate the C-IDA sensing mechanism for the detection of anionic, cationic, and neutral analytes via prominent UV-vis spectral changes have been revealed. Besides, the recognition of several biologically essential analytes viz. phosphate and saccharides have also been discussed in detail through numerous examples. The authors believe that this chapter on C-IDA will offer new dimensions to the design and fabrication of IDA-based colorimetric sensors.


Keywords: Absorption, Analyte, Binding, Binding affinity, Colorimetric indicator displacement assay, Chemosensor, Chromophore, Colorimetric indicator, Colorimetric sensor, Calorimetric titration, Detection, Isosbestic, Naked eye, Quantification, Receptor, Sensing ensemble, Stoichiometry, Signal transduction, Sensing, Saccharides.

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