Diseases caused by pathogenic microbes have been a serious problem since
decades. They often cause high mortality in both developing and developed countries.
Diseases including diarrhea, cholera, typhoid, etc., are often involved in outbreaks
related to pathogens surviving in water. In recent years, the management of infectious
diseases has drawn much attention among scientists and researchers. Various specific,
sensitive and reproducible detection methods have been developed to identify pathogen
contamination in water. These advanced methods generally use probes for diagnostics
of pathogens. The present chapter attempts to focus on the development of various
probe chemistries for the detection of pathogens. We also intend to present here the
pros and cons of different methods.
Keywords: Amplification, Annealing, Culture, Enumeration, Fluorescence, Infectious disease, Microscopic examination, Molecular Techniques, Mortality, Outbreaks, PCR, Probe, Quantification, Quencher, Real Time PCR, SYBR GREEN1, Sensitivity, Specificity, Template, Target.