Owing to the incidence of diabetes, glucose sensing in diabetes diagnosis and therapy is of great significance. In addition, in the drug and food sectors, glucose sensing is also important. Via different techniques, such as electrochemical or optical approaches, glucose sensing has been achieved. Sensors play an important role in the identification of chemical and biological samples and have attracted a great deal of interest in recent decades. Signals are produced by the binding of the analytical sensor. Varieties of chemical sensor, including cationic and anionic sensors, are used. In chemical sensing, molecular recognition and molecular transduction exist. There are three pieces of a chemo sensor, the binding site receptor, the device whose properties change with the binding and the spacer. The advanced glucose sensors development with high sensitivity and suitability has been facilitated by novel transducers made with nanomaterials that combine fluorescent methods. Glucose detection by a chemo sensor is discussed in this chapter. In addition, techniques for combining biological sensing and fluorescent nanomaterials components are explored, and the applicability of the chemosensor is also illustrated, making it suitable for glucose sensing. It is concluded that the extensive use of chemosensors in the health care sector makes them convenient instruments for real-time identification and long-term tracking of the environmental, biological and physical state of the human body.