Generic placeholder image

Recent Patents on Anti-Infective Drug Discovery


ISSN (Print): 1574-891X
ISSN (Online): 2212-4071

Research Article

Identification of Quinolone and Colistin Resistance Genes in Escherichia Coli Strains Isolated from Mucosal Samples of Patients with Colorectal Cancer and Healthy Subjects

Author(s): Hassan Mahmoudi, Sima Ghiasvand, Omid Zarei, Hadi Hossainpour and Mohammad Y. Alikhani*

Volume 15 , Issue 1 , 2020

Page: [30 - 40] Pages: 11

DOI: 10.2174/1574891X14666190611125951


Introduction: Antibiotic resistance and extensive use of antibiotics are amongst the major causes of failure in antibiotic treatment. The purpose of this study was to investigate antibiotic resistance patterns and to identify resistance genes of quinolones and colistin in Escherichia coli. There are a very few patents on E. coli isolated from colorectal cancer. So, this study demonstrates that some bacteria resistant to ciprofloxacin have not resistance genes.Moreover, new patterns for E. coli are presented for isolates of patients with colorectal cancer.

Materials and Methods: Of the three healthy people, inflammatory bowel diseases (IBD) patients and colorectal cancer patients, 40 E. coli strains isolated after confirmation by biochemical and molecular methods. The susceptibility of isolates to antibiotics was investigated using disk diffusion test. After deoxyribonucleic acid (DNA) extraction, polymerase chain reaction (PCR) was used to identify genes encoding resistance to ciprofloxacin (qnr A, qnr B) and colistin (mcr-1).

Results: The results showed that E. coli isolates from colorectal cancer patients had the highest resistance to piperacillin (67.5%), ceftazidime (47.5%), and cefepime (42.5%). Also, E. coli strains isolated from IBD patients showed resistance to antibiotic ceftazidime 13%. More than 95% of E. coli strains isolated from healthy people were susceptible to antibiotics. Based on the results, 18 (15%) E. coli strains showed resistance to ciprofloxacin. The qnr A gene was detected in 61.11% isolates; however, qnr B was detected in 9 (50%) isolates. Isolates resistant to colistin were not observed.

Conclusion: These findings indicate increased resistance of E. coli to ciprofloxacin in comparison with prior studies. Further research in this field will increase our knowledge and more effective exposure to the antibiotic resistance of the pathogenic microorganisms.

Keywords: Escherichia coli, ciprofloxacin, colistin, antibiotic resistance, colorectal cancer, inflammatory bowel diseases.

Graphical Abstract
Nordmann P, Cuzon G, Naas T. The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. Lancet Infect Dis 2009; 9(4): 228-36.
[] [PMID: 19324295]
Patel JB, Rasheed JK, Kitchel B. Carbapenemases in enterobacteriaceae: activity, epidemiology, and laboratory detection. Clin Microbiol Newsl 2009; 31(8): 55-62.
Paterson DL, Bonomo RA. Extended-spectrum β-lactamases: a clinical update. Clin Microbiol Rev 2005; 18(4): 657-86.
[] [PMID: 16223952]
Bonnet M, Buc E, Sauvanet P, et al. Colonization of the human gut by E. coli and colorectal cancer risk. Clin Cancer Res 2014; 20(4): 859-67.
[] [PMID: 24334760]
Antonic V, Stojadinovic A, Kester KE, et al. Significance of infectious agents in colorectal cancer development. J Cancer 2013; 4(3): 227-40.
[] [PMID: 23459622]
Zur Hausen H. The search for infectious causes of human cancers: where and why. Virology 2009; 392(1): 1-10.
[] [PMID: 19720205]
Dalton-Griffin L, Kellam P. Infectious causes of cancer and their detection. J Biol 2009; 8(7): 67.
[] [PMID: 19678917]
Zhu Q, Gao R, Wu W, Qin H. The role of gut microbiota in the pathogenesis of colorectal cancer. Tumour Biol 2013; 34(3): 1285-300.
[] [PMID: 23397545]
Buc E, Dubois D, Sauvanet P, et al. High prevalence of mucosa-associated E. coli producing cyclomodulin and genotoxin in colon cancer. PLoS One 2013; 8(2)e56964
[] [PMID: 23457644]
Serfaty L, De Leusse A, Rosmorduc O, et al. Ursodeoxycholic acid therapy and the risk of colorectal adenoma in patients with primary biliary cirrhosis: an observational study. Hepatology 2003; 38(1): 203-9.
[] [PMID: 12830003]
CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-eight Informational Supplement. CLSI document M100-S28 Clinical and Laboratory Standards Institute 2018.
Robicsek A, Strahilevitz J, Sahm DF, Jacoby GA, Hooper DC. qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States. Antimicrob Agents Chemother 2006; 50(8): 2872-4.
[] [PMID: 16870791]
Liu Y-Y, Wang Y, Walsh TR, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016; 16(2): 161-8.
[] [PMID: 26603172]
Aibinu IE, Adenipekun EO, Nwaka D, Adelowotan AO, Ajekigbe A, Adeyemi O. Emergence of cross-resistance to fluoroquinolones in gram-negative isolates from cancer infections in a tertiary hospital in Nigeria. J Am Sci 2008; 4: 14-2.
Kheirabadi SA, Najafipour S, Kafilzadeh F, Abdollahi A, Jafari S, Moravej A. Evaluation of drug resistance pattern of Escherichia coli strains isolated from Fasa Vali-e-Asr hospital patients. Journal of Fasa University of Medical Sciences 2013; 2(4): 273-8.
Mansory Jamshidi N. Pakzad e, Tabaraee b, Hadadi a. Frequency of qnr genes in Escherichia coli strains resistant to quinolones isolated from Ilam Imam Khomani hospital and Tehran Milad hospital. Sci J Ilam Univ Med Sci 2013; 21(6): 16-22.
Heisig P, Schedletzky H, Falkenstein-Paul H. Mutations in the gyrA gene of a highly fluoroquinolone-resistant clinical isolate of Escherichia coli. Antimicrob Agents Chemother 1993; 37(4): 696-701.
[] [PMID: 8388197]
Chapman JS, Georgopapadakou NH. Routes of quinolone permeation in Escherichia coli. Antimicrob Agents Chemother 1988; 32(4): 438-42.
[] [PMID: 3132091]
Conrad S, Oethinger M, Kaifel K, Klotz G, Marre R, Kern WV. GyrA mutations in high-level fluoroquinolone-resistant clinical isolates of Escherichia coli. J Antimicrob Chemother 1996; 38(3): 443-55.
[] [PMID: 8889719]
Ruiz J. Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection. J Antimicrob Chemother 2003; 51(5): 1109-17.
[] [PMID: 12697644]
Siasi E, Hossieni F, Rahiminia P. Relation of gyrA and parC genes mutations with fluoroquinolonesresistance in Escherichia coli of urinary tract infections. New Cell Mol Biotechnol J 2017; 7(25): 91-100.
Moreno E, Prats G, Sabaté M, Pérez T, Johnson JR, Andreu A. Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli. J Antimicrob Chemother 2006; 57(2): 204-11.
[] [PMID: 16390858]
Muhammad I, Uzma M, Yasmin B, Mehmood Q, Habib B. Prevalence of antimicrobial resistance and integrons in Escherichia coli from Punjab, Pakistan. Braz J Microbiol 2011; 42(2): 462-6.
[] [PMID: 24031655]
Sanchez GV, Master RN, Karlowsky JA, Bordon JM. In vitro antimicrobial resistance of urinary E. coli among US outpatients from 2000 to 2010. Antimicrob Agents Chemother 2012; 56(4): 2181-3.
Mohajeri P, Izadi B, Rezai M, Falahi B, Khademi H, Ebrahimi R. Assessment of the frequency of extended spectrum beta lactamases producing Escherichia coli isolated from urinary tract infections and its antibiotic resistance pattern in kermanshah. J Ardabil Univ Med Sci 2011; 11(1): 86-94.
Farshad S, Ranjbar R, Anvarinejad M, Shahidi MA, Hosseini M Eds. Emergence of multi drug resistant strains of Escherichia coli isolated from urinary tract infection. Open Conf Proc J 2010; 1: 192-6.
Jabeen K, Zafar A, Hasan R. Frequency and sensitivity pattern of extended spectrum beta lactamase producing isolates in a tertiary care hospital laboratory of Pakistan. J Pak Med Assoc 2005; 55(10): 436-9.
[PMID: 16304852]
Poole K. Resistance to β-lactam antibiotics. Cell Mol Life Sci 2004; 61(17): 2200-23.
[] [PMID: 15338052]
Zhou T-L, Chen X-J, Zhou M-M, Zhao Y-J, Luo X-H, Bao Q-Y. Prevalence of plasmid-mediated quinolone resistance in Escherichia coli isolates in Wenzhou, Southern China, 2002-2008. Jpn J Infect Dis 2011; 64(1): 55-7.
[PMID: 21266756]
Akya A, Chegenelorestani R, Elahi A, Hamzavi Y. Frequency of plasmid-mediated quinolone resistance genes in extended-spectum β-lactamase-producing Escherichia coli. Majallah-i Danishgah-i Ulum-i Pizishki-i Mazandaran 2017; 27(151): 41-51.
Bouchakour M, Zerouali K, Gros Claude JD, et al. Plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing enterobacteriaceae in Morocco. J Infect Dev Ctries 2010; 4(12): 779-803.
[PMID: 21252459]
Rajaei S, Kazemi-Pour N, Rokhbakhsh-Zamin F. Frequency of plasmid-mediated quinolone resistance genes among clinical isolates of Pseudomonas aeruginosa in Kerman, Iran. Indian J Med Microbiol 2017; 11(3): 10-8.
Soleimani-Asl Y, Zibaei M, Firoozeh F. Detection of qnrA gene among quinolone-resistant Escherichia coli isolated from urinary tract infections in Khorram Abad during 2011-2012. Feyz J Kashan Univ Med Sci 2013; 17(5): 488-94.
Corkill JE, Anson JJ, Hart CA. High prevalence of the plasmid-mediated quinolone resistance determinant qnrA in multidrug-resistant Enterobacteriaceae from blood cultures in Liverpool, UK. J Antimicrob Chemother 2005; 56(6): 1115-7.
[] [PMID: 16260446]
Oktem IMA, Gulay Z, Biçmen M, Gur D, Group HPS. qnrA prevalence in extended-spectrum beta-lactamase-positive Enterobacteriaceae isolates from Turkey. Jpn J Infect Dis 2008; 61(1): 13-7.
[PMID: 18219128]
Wang M, Tran JH, Jacoby GA, Zhang Y, Wang F, Hooper DC. Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China. Antimicrob Agents Chemother 2003; 47(7): 2242-8.
[] [PMID: 12821475]
Pakzad I, Ghafourian S, Taherikalani M, et al. Qnr prevalence in extended spectrum beta-lactamases (ESBLs) and none-ESBLs producing Escherichia coli isolated from urinary tract infections in central of Iran. Iran J Basic Med Sci 2011; 14(5): 458-64.
[PMID: 23493061]
Mohamadbigi M, Akbarmehr J, Jafari B. Evaluation of the frequency of plasmid-mediated quinolone resistance genes in clinical isolates of Escherichia coli and Klebsiella spp in Tehran 2016. J MICRO WORLD 2016; 9: 199-207.
Bansal S, Tandon V. Contribution of mutations in DNA gyrase and topoisomerase IV genes to ciprofloxacin resistance in Escherichia coli clinical isolates. Int J Antimicrob Agents 2011; 37(3): 253-5.
[ 2010.11.022] [PMID: 21236644]
Shin JH, Jung HJ, Lee JY, Kim HR, Lee JN, Chang CL. High rates of plasmid-mediated quinolone resistance QnrB variants among ciprofloxacin-resistant Escherichia coli and Klebsiella pneumoniae from urinary tract infections in Korea. Microb Drug Resist 2008; 14(3): 221-6.
[] [PMID: 18707554]
Mirmostafa S, Sabet M, Amir N. Determination of plasmid profile and antibiotic resistance pattern in clinical Escherichia coli strains, isolated from different region of Karaj city. New Cell Mol Biotechnol J 2014; 3(12): 93-7.
Yosofi S, Mojtahedi A, Shenaghari M. Association to qnr genes with ciprofloxacin resistant E. coli. Kordestan Univ Med Sci 2015; 20(5): 52-60.

© 2022 Bentham Science Publishers | Privacy Policy