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Current Pharmaceutical Analysis

Editor-in-Chief

ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Research Article

Determination of Shanzhiside Methylester in Rat Plasma by Uplc-Ms/Ms and its Application to a Pharmacokinetic Study

Author(s): Qinghua Weng, Yichuan Chen, Zuoquan Zhong, Qianqian Wang, Lianguo Chen and Xianqin Wang*

Volume 16, Issue 7, 2020

Page: [960 - 966] Pages: 7

DOI: 10.2174/1573412915666190329215736

Price: $65

Abstract

Introduction: In this study, we used UPLC-MS/MS to detect shanzhiside methylester in rat plasma, and investigated its pharmacokinetics in rats.

Materials and Methods: Diazepam was utilized as an internal standard (IS), and acetonitrile precipitation method was used to process the plasma samples. Chromatographic separation was achieved using a UPLC BEH C18 column using mobile phase of methanol-0.1 % formic acid with gradient elution. Electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive ionization was applied.

Results: The results indicated that within the range of 5-4000 ng/mL, linearity of shanzhiside methylester in rat plasma was acceptable (r>0.995), and the lower limit of quantification (LLOQ) was 5 ng/mL. Intra-day and inter-day precision RSD of shanzhiside methylester in rat plasma were lower than 14%. Accuracy range was between 87.3 % and 109.1 %, and matrix effect was between 99.2% and 106.3%.

Conclusion: The method was successfully applied in the pharmacokinetics of shanzhiside methylester in rats after intravenous administration.

Keywords: Shanzhiside methylester, pharmacokinetics, rat, UPLC-MS/MS, plasma, Diazepam.

Graphical Abstract
[1]
Li, M.; Jia, Z.; Zhang, R. A review on the study of Lamiophlomis rotata(Benth.) Kudo: an analgesic and hemostatic drug. Zhong Yao Cai, 2004, 27(3), 222-224.
[PMID: 15346587]
[2]
Li, M.; Zhang, R.; Jia, Z.; Sheng, J.; Qiu, J.; Wang, J. Isolation and identification of hemostatic ingredients from Lamiophlomis rotata (Benth.) Kudo. Phytother. Res., 2009, 23(6), 816-822.
[http://dx.doi.org/10.1002/ptr.2669] [PMID: 19173219]
[3]
Li, M.; Shang, X.; Zhang, R.; Jia, Z.; Fan, P.; Ying, Q.; Wei, L. Antinociceptive and anti-inflammatory activities of iridoid glycosides extract of Lamiophlomis rotata (Benth.) Kudo. Fitoterapia, 2010, 81(3), 167-172.
[http://dx.doi.org/10.1016/j.fitote.2009.08.018] [PMID: 19698769]
[4]
Fan, H.; Li, T.F.; Gong, N.; Wang, Y.X. Shanzhiside methylester, the principle effective iridoid glycoside from the analgesic herb Lamiophlomis rotata, reduces neuropathic pain by stimulating spinal microglial β-endorphin expression. Neuropharmacology, 2016, 101, 98-109.
[http://dx.doi.org/10.1016/j.neuropharm.2015.09.010] [PMID: 26363192]
[5]
Que, WL; Lu, SM; Zhang, KN; Yang, T; Liao, LD; Deng, Y. detection method of lamiophlomis rotata extract, medicine composition containing the extract. cn patent 101,984,983b, 2013. March 13,
[6]
Zhong, S.H.; Gu, R.; Wang, L.X.; Liao, Y.F.; Zheng, X.H.; Zheng, X.; Cao, Y. Simultaneous determination of seven constituents in Lamiophlomis rotata by HPLC. Zhongguo Zhongyao Zazhi, 2014, 39(22), 4373-4378.
[PMID: 25850270]
[7]
Fan, P.C.; Li, M.X.; Ma, H.P.; Jing, L.L.; Qiu, J.G.; Jia, Z.P. Simultaneous determination of four shanzhiside methylester derivatives in rabbit plasma by liquid chromatography/tandem mass spectrometry. Biomed. Chromatogr., 2012, 26(12), 1543-1551.
[http://dx.doi.org/10.1002/bmc.2730] [PMID: 22422345]
[8]
Chen, J.; Wang, Y.; Liang, X.; Sun, T.; Luo, J.; Guo, X.; Zhao, L. Simultaneous determination of shanzhiside methyl ester, 8-Oacetylshan- zhiside methyl ester and luteolin-7-O-β-Dglucopyranoside in rat plasma by ultra-performance liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study after oral administration of Lamiophlomis rotata Pill. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2016, 1020, 62-66.
[http://dx.doi.org/10.1016/j.jchromb.2016.03.021] [PMID: 27023158]
[9]
Lu, W.; Bennett, B.D.; Rabinowitz, J.D. Analytical strategies for LC-MS-based targeted metabolomics. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2008, 871(2), 236-242.
[http://dx.doi.org/10.1016/j.jchromb.2008.04.031] [PMID: 18502704]
[10]
Zhou, Y.F.; Chen, B.B.; Chen, J.Y.; Dong, Y.W.; Wang, S.H.; Wen, C.C.; Wang, X.Q.; Yu, X.M. Determination and pharmacokinetic study of jaceosidin in rat plasma by UPLC-MS/MS. Acta Chromatogr., 2018, 30, 131-135.
[http://dx.doi.org/10.1556/1326.2017.00104]
[11]
Ye, W.; Lin, X.; Zhang, Y.; Xu, Y.; Sun, R.; Wen, C.; Wang, X.; Bao, S.; Chen, R. Quantification and pharmacokinetics of alpinetin in rat plasma by UHPLC-MS/MS using protein precipitation coupled with dilution approach to eliminate matrix effects. J. Pharm. Biomed. Anal., 2018, 152, 242-247.
[http://dx.doi.org/10.1016/j.jpba.2017.12.046] [PMID: 29433096]
[12]
Wu, H.; Yan, Q.; Fan, Z.; Huang, M.; He, J.; Ma, J.; Wang, X. Determination of corypalmine in mouse blood by UPLC-MS/MS and its application to a pharmacokinetic study. Biomed. Chromatogr., 2018, 32(8) e4255
[http://dx.doi.org/10.1002/bmc.4255] [PMID: 29633295]
[13]
Wei, Z.; Ye, L.X.; Jiang, Y.Y.; Zhang, Z.G.; Wang, X.Q. Determination of Saikosaponin B1 and Saikosaponin B3 in Rat Plasma by UPLC-MS/MS and its application to a pharmacokinetic study. Lat. Am. J. Pharm., 2018, 37, 523-528.
[14]
Chen, L.; Zhang, B.; Liu, J.; Fan, Z.; Weng, Z.; Geng, P.; Wang, X.; Lin, G. Pharmacokinetics and bioavailability study of monocrotaline in mouse blood by ultra-performance liquid chromatographytandem mass spectrometry. BioMed Res. Int., 2018. 20181578643
[http://dx.doi.org/10.1155/2018/1578643] [PMID: 30186850]
[15]
Ye, W.; Chen, R.; Sun, W.; Huang, C.; Lin, X.; Dong, Y.; Wen, C.; Wang, X. Determination and pharmacokinetics of engeletin in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2017, 1060, 144-149.
[http://dx.doi.org/10.1016/j.jchromb.2017.06.018] [PMID: 28622617]
[16]
Wang, X.Q.; Wang, Q.Q.; Hu, Q.P.; Zhao, Y.; Chen, W.H.; Wen, C.C.; Wu, B. Effect of ethanol on pharmacokinetics of ketamine in Rat. Lat. Am. J. Pharm., 2017, 36, 1403-1407.
[17]
Wang, S.; Dong, Y.; Su, K.; Zhang, J.; Wang, L.; Han, A.; Wen, C.; Wang, X.; He, Y. Effect of codeine on CYP450 isoform activity of rats. Pharm. Biol., 2017, 55(1), 1223-1227.
[http://dx.doi.org/10.1080/13880209.2017.1297466] [PMID: 28253826]
[18]
Geng, P.W.; Cai, J.Z.; Zhang, L.J.; Xu, M.Z.; Liu, Z.Z.; Zhang, J.; Wang, Z.Y.; Wang, X.Q.; Wen, C.C.; Ma, J.S. Liver tissue metabonomics in rat after acute paraquat poisoning gas chromatography-mass spectrometry. Int. J. Clin. Exp. Med., 2017, 10, 937-943.
[19]
Chen, L.G.; Su, K.; Jiang, Y.Y.; Chen, B.B.; Wen, C.C.; Zeng, M.; Wang, X.C. Pharmacokinetic study of fenofibrate in rat by high performance liquid chromatography. Lat. Am. J. Pharm., 2016, 35, 1450-1453.
[20]
Ma, J.; Wang, S.; Huang, X.; Geng, P.; Wen, C.; Zhou, Y.; Yu, L.; Wang, X. Validated UPLC-MS/MS method for determination of hordenine in rat plasma and its application to pharmacokinetic study. J. Pharm. Biomed. Anal., 2015, 111, 131-137.
[http://dx.doi.org/10.1016/j.jpba.2015.03.032] [PMID: 25880244]
[21]
Zhang, Q.; Wen, C.; Xiang, Z.; Ma, J.; Wang, X. Determination of CUDC-101 in rat plasma by liquid chromatography mass spectrometry and its application to a pharmacokinetic study. J. Pharm. Biomed. Anal., 2014, 90, 134-138.
[http://dx.doi.org/10.1016/j.jpba.2013.11.031] [PMID: 24366214]

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