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Combinatorial Chemistry & High Throughput Screening


ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

Research Article

Computational Method for the Identification of Molecular Metabolites Involved in Cereal Hull Color Variations

Author(s): Yunhua Zhang, Dong Dong, Dai Li, Lin Lu, JiaRui Li, YuHang Zhang and Lijuan Chen*

Volume 21 , Issue 10 , 2018

Page: [760 - 770] Pages: 11

DOI: 10.2174/1386207322666190129105441

Price: $65


Background: Cereal hull color is an important quality specification characteristic. Many studies were conducted to identify genetic changes underlying cereal hull color diversity. However, these studies mainly focused on the gene level. Recent studies have suggested that metabolomics can accurately reflect the integrated and real-time cell processes that contribute to the formation of different cereal colors.

Methods: In this study, we exploited published metabolomics databases and applied several advanced computational methods, such as minimum redundancy maximum relevance (mRMR), incremental forward search (IFS), random forest (RF) to investigate cereal hull color at the metabolic level. First, the mRMR was applied to analyze cereal hull samples represented by metabolite features, yielding a feature list. Then, the IFS and RF were used to test several feature sets, constructed according to the aforementioned feature list. Finally, the optimal feature sets and RF classifier were accessed based on the testing results.

Results and Conclusion: A total of 158 key metabolites were found to be useful in distinguishing white cereal hulls from colorful cereal hulls. A prediction model constructed with these metabolites and a random forest algorithm generated a high Matthews coefficient correlation value of 0.701. Furthermore, 24 of these metabolites were previously found to be relevant to cereal color. Our study can provide new insights into the molecular basis of cereal hull color formation.

Keywords: Cereal hull color, molecular metabolites, minimum redundancy maximum relevance, random forest, incremental forward search.

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