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Current Nutrition & Food Science

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ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

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Research Article

Production of Rhamnolipid Biosurfactant from Fed Batch Culture by Pseudomonas aeruginosa using Multiple Substrates

Author(s): Jujjavarapu S. Eswari*

Volume 16, Issue 6, 2020

Page: [928 - 933] Pages: 6

DOI: 10.2174/1573401314666181107100127

Price: $65

Abstract

Objective: Biosurfactants are the surface active agents which are used for the reduction of surface and interfacial tensions of liquids. Rhamnolipids are the surfactants produced by Pseudomonas aeruginosa. It requires minimum nutrition for its growth as it can also grow in distilled water. The rhamnolipids produced by Pseudomonas aeruginosa are extra-cellular glycolipids consisting of L-rhamnose and 3-hydroxyalkanoic acid.

Methods: The fed-batch method for the rhamnolipid production is considered in this study to know the influence of the carbon, nitrogen, phosphorous substrates as growth-limiting nutrients. Pulse feeding is employed for limiting nutrient addition at particular time interval to obtain maximum rhamnolipid formation from Pseudomonas aeruginosa compared with the batch process.

Results: Out of 3 fed batch strategies constant glucose fed batch strategy shows best and gave maximum rhamnolipid concentration of 0.134 g/l.

Keywords: Fed-batch fermentation, kinetics, pseudomonas aeruginosa, rhamnolipids, growth, substrate.

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[1]
Prieto LM, Michelon M, Burkert JFM, Kalil SJ, Burkert CAV. The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil. Chemosphere 2008; 71(9): 1781-5.
[http://dx.doi.org/10.1016/j.chemosphere.2008.01.003] [PMID: 18289632]
[2]
Wei YH, Chou JL, Chang JS. Rhamnolipid production by an indigenous isolate Pseudomonas aeruginosa J4 originating from petrochemical wastewater. Biochem Eng J 2005; 27(2): 146-54.
[http://dx.doi.org/10.1016/j.bej.2005.08.028]
[3]
Desai JD, Banat IM. Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 1997; 61(1): 47-64.
[http://dx.doi.org/10.1128/.61.1.47-64.1997] [PMID: 9106364]
[4]
Homayoon B, Fazaelipoor MH, Schaffie M. Isolation of a biosurfactant producing microorganism, kinetic experiments, and separation of the biosurfactant from liquid culture. J Sep Sci Eng 2010; 2: 13.
[5]
Satya Eswari J, Mohan A, Venkateswarlu C. Optimum Culture medium composition for rhamnolipid production by Pseudomonas aeruginosa AT10 using a novel multiobjective optimization method. J Chem Technol Biotechnol 2013; 88(2): 271-9.
[http://dx.doi.org/10.1002/jctb.3825]
[6]
Harvey S, Elashvili I, Valdes JJ, Kamely D, Chakrabarty AM. Enhanced removal of Exxon Valdez spilled oil from Alaskan gravel by a microbial surfactant. Biotechnology (N Y) 1990; 8(3): 228-30.
[PMID: 1367420]
[7]
Banat IM, Makkar RS, Cameotra SS. Potential commercial applications of microbial surfactants. Appl Microbiol Biotechnol 2000; 53(5): 495-508.
[http://dx.doi.org/10.1007/s002530051648] [PMID: 10855707]
[8]
Rodrigues L, Teixeira J, Oliveira R, van der Mei HC. Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria. Process Biochem 2006; 41: 1-10.
[http://dx.doi.org/10.1016/j.procbio.2005.01.030]
[9]
Francy DS, Thomas JM, Raymond RL, Ward CH. Emulsification of hydrocarbons by subsurface bacteria. J Ind Microbiol 1991; 8: 237-46.
[http://dx.doi.org/10.1007/BF01576061]
[10]
Guerra-Santos L, Käppeli O, Fiechter A. Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source. Appl Environ Microbiol 1984; 48(2): 301-5.
[http://dx.doi.org/10.1128/AEM.48.2.301-305.1984] [PMID: 6435520]
[11]
Benincasa M, Contiero J, Manresa MA, Moraes IO. Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soapstock as the sole carbon source. J Food Eng 2002; 54: 283-8.
[http://dx.doi.org/10.1016/S0260-8774(01)00214-X]
[12]
Rikalovic MG, Gjgic G, Karadzic I. Production and characterization of Rhamnolipids from Pseudomonas aeruginosa sanai. J Serb Chem Soc 2002; 77(1): 27-42.
[13]
Lee KM, Hwang SH, Ha SD, Jang JH, Lim DJ, Kong JY. Rhamnolipid production in batch and fedbatch fermentation using Pseudomonas aeruginosa BYK2. Biotechnol Bioprocess Eng 2004; 9: 267-73.
[http://dx.doi.org/10.1007/BF02942342]
[14]
Noh NAM, Salleh SM, Abdullah AAA, Yahya ARM. Fed-batch Cultivation of Pseudomonas aeruginosa. International Conference on Biological and Life Sciences 2012.
[15]
Zhao X, Hu C, Wu S, Shen H, Zhao ZK. Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies. J Ind Microbiol Biotechnol 2011; 38(5): 627-32.
[http://dx.doi.org/10.1007/s10295-010-0808-4] [PMID: 20711796]
[16]
Zhang L, Yang Y, Sun J, et al. Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30. Bioresour Technol 2010; 101(6): 1961-7.
[http://dx.doi.org/10.1016/j.biortech.2009.10.052] [PMID: 19932023]

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