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Current Pharmaceutical Design

Editor-in-Chief

ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

General Review Article

Aspects of Drug-Protein Binding and Methods of Analyzing the Phenomenon

Author(s): Karolina Wanat*, Elżbieta Brzezińska and Anna W. Sobańska

Volume 24 , Issue 25 , 2018

Page: [2974 - 2985] Pages: 12

DOI: 10.2174/1381612824666180808145320

Price: $65

Abstract

In recent decades, drug-protein interactions have been widely studied and several methods of analysis of these phenomena have been developed and improved. These can be classified into separation, physical, chromatographic and electrophoretic methods. This review depicts the assumptions and mechanisms of methods from each group, details their strengths and weaknesses, and presents examples of their usage from the literature. Equilibrium dialysis, ultrafiltration, Hummel-Dreyer method or high performance affinity chromatography are given as representative examples, but this issue is far more expanded. Nowadays, increasing attention is paid to the computational methods and molecular modeling which are convenient tools to estimate protein binding affinity based on the physicochemical properties of compounds. To gain a broader overview, the study also examines the protein binding ability and pharmacotherapy of drugs against a range of substrates such as plasma, skin, tissue and human milk.

Keywords: Drug-protein binding, protein binding analysis, computational modeling, human serum albumin, alpha-1-acid glycoprotein, quantitative structure-activity relationship.


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