Abstract
Background: Various aliphatic polyesters recently developed as biodegradable plastics are potentially useful as bioresorbable polymers for biomedical application. In implanting biodegradable devices in vivo, it is necessary to evaluate their biocompatibility, histocompatibility, and hemocompatibility.
Objective: The relationship between the cell adhesion and proliferation and the protein adsorption on three kinds of biodegradable aliphatic polyesters, poly(L-lactide) (PLLA), poly(3-[R]-hydroxybutyrate/ valerate) ([R]-PHB/HV) and poly(butylene succinate-co-lactide) (PBSL) was investigated and compared with that on [RS]-PHB.
Methods: The cell adhesion was evaluated with 3T3-L1 fibroblast, HeLa S3, and HUVECs (human umbilical vein endothelial cells) cells on a polymer film of each polyester before and after protein adsorption.
Results: The cells attached to the films of PLLA, PBSL and [R]-PHB/HV, whereas the cells attached to [RS]-PHB kept a round shape. The cell adhesion of HeLa S3 was found to be similar to that of 3T3- L1 fibroblast cells, decreasing in the order of PLLA>PBSL>[R]-PHB/HV>[RS]-PHB. The optical micrographs of 3T3-L1 fibroblast and HeLa S3 cells attached on the film surfaces revealed that the cells were activated to undergo proliferation and extension of all the polymer films except the [RS]- PHB film. The HUVEC cells were also activated to induce proliferation and extension on the PLLA and PBSL films.
Conclusion: The three cells were highly activated on each of the polyester films to undergo cell proliferation and extension, being different from the behavior of cells on [RS]-PHB. These data indicated that the biodegradable polyesters have excellent biocompatibility for use as scaffold materials in tissue engineering.
Keywords: 3T3-L1 fibroblast, biocompatibility, biodegradable aliphatic polyester, biomedical material, cell adhesion, HeLa S3, histocompatibility, HUVEC.
Graphical Abstract
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