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Current Pharmaceutical Analysis


ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Research Article

Development of an HPLC-UV Method for Quantification of Stattic

Author(s): Soheil Sedaghat, Ommoleila Molavi, Akram Faridi, Ali Shayanfar* and Mohammad Reza Rashidi

Volume 15, Issue 6, 2019

Page: [568 - 573] Pages: 6

DOI: 10.2174/1573412914666180523092957

Price: $65


Background: Signal transducer and activator of transcription 3 (STAT3), an oncogenic protein found constitutively active in many types of human malignancies, is considered to be a promising target for cancer therapy.

Objective: In this study for the first time, a simple and accurate method has been developed for the determination of a STAT3 dimerization inhibitor called stattic in aqueous and plasma samples.

Methods: A reverse-phase high-performance liquid chromatography (RP-HPLC) composed of C18 column as stationary phase, and the mixture of acetonitrile (60%) and water (40%) as mobile phase with a UV detection at 215 nm were applied for quantification of stattic. The developed method was validated by Food and Drug Administration (FDA) guideline.

Results: The method provided a linear range between 1-40 and 2.5-40 µg mL-1 for aqueous and plasma samples, respectively, with a correlation coefficient of 0.999. The accuracy (as recovery) of the developed method was found to be between 95-105% for aqueous medium and 85-115% for plasma samples. The precision (as relative standard deviation) for aqueous and plasma samples was less than 6% and 15%, respectively. The sensitivity of the developed method based on FDA guideline was 1 µg mL-1 for aqueous and 2.5 µg mL-1 for plasma samples.

Conclusion: These results show that the established method is a fast and accurate quantification for stattic in aqueous and plasma samples.

Keywords: Analysis method, cancer, HPLC-UV, plasma, stattic, validation.

Graphical Abstract
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