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Combinatorial Chemistry & High Throughput Screening

Editor-in-Chief

ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

Fluorescence Activated Cell Sorting for Enzymatic Activity

Author(s): Edgardo T. Farinas

Volume 9 , Issue 4 , 2006

Page: [321 - 328] Pages: 8

DOI: 10.2174/138620706776843200

Price: $65

Abstract

Directed evolution is a reliable method for protein engineering and as a tool for investigating structure/function relationships. A key for a successful directed evolution experiment is oftentimes the screen. Fluorescence activated cell sorting (FACS) is powerful high-throughput screening approach to isolate and identify mutants from large protein libraries. FACS has been successful in isolating proteins with improved or altered binding affinity. However, FACS screening for mutants with enhanced catalytic activity has been met with limited success. This review focuses on the FACS screening of protein libraries for enzymatic activity.

Keywords: Directed evolution, FACS, high-throughput screening, enzyme engineering


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