Averrhoa bilimbi L. leaf Aqueous Extract Modulates both Cardiac Contractility and Frequency in the Guinea-pig Atrium by the Activation of Muscarinic Receptors

Title:Averrhoa bilimbi L. leaf Aqueous Extract Modulates both Cardiac Contractility and Frequency in the Guinea-pig Atrium by the Activation of Muscarinic Receptors

Volume: 15 Issue: 11

Author(s): Anne Caroline Oliveira dos Santos, Diego Santos Souza, Thassio Ricardo Ribeiro Mesquita, Jose Evaldo Rodrigues de Menezes-Filho, Ana Paula Duarte Caldas, Americo Azevedo de Souza, Gilmara Beatriz Andrade da Silva, Evaleide Diniz de Oliveira, Leonardo Rodrigues dos Santos and Carla Maria Lins de Vasconcelos*

Affiliation:

• Laboratory of Heart Biophysics, Department of Physiology, Federal University of Sergipe, Marechal Rondon, s/n, Jardim Rosa Elze, 49100-000, São Cristovao, SE,Brazil

Keywords: Averrhoa bilimbi, atrium, guinea pig, contractility, heart rate, muscarinic receptor.

Abstract: Background: Averrhoa bilimbi has been used in the traditional medicine for treatment of hypertension, diabetes and as an antihypercholesterolemic. This study investigated the inotropic and chronotropic effects of Averrhoa bilimbi aqueous extract (AqE) on the guinea pig atrium.

Methods: Left and right atria isolated from guinea pig were used to evaluate the atrial contractility and atrial rate, respectively.

Results: In left atria, AqE showed a negative inotropic effect and delaying the time to peak without change relaxation time. In right atria, AqE reduced the atrial rate (67%). Furthermore, AqE induced a rightward shift in the concentration-effect curve of CaCl2 and reduced by 61% the Bowditch phenomenon. To evaluate the mechanism involved in the effects evoked by AqE, concentration-effect curves were obtained in the presence of atropine (M2 muscarinic receptor antagonist) and TEA (non-selective potassium channels blocker). The results revealed that these antagonists shifted rightward the concentration-effect curve of AqE.

Conclusion: In conclusion, AqE reduces both atrial contractility and heart rate by activation of M2 receptors increasing potassium efflux associated with decreased calcium entry in cardiomyocyte. These effects are important for its future pharmacological applications being a source of new drugs that can be use in treatment of cardiovascular disease.

Cite this article as:

Oliveira dos Santos Caroline Anne, Souza Santos Diego, Ribeiro Mesquita Ricardo Thassio, Rodrigues de Menezes-Filho Evaldo Jose, Duarte Caldas Paula Ana, de Souza Azevedo Americo, Andrade da Silva Beatriz Gilmara, de Oliveira Diniz Evaleide, dos Santos Rodrigues Leonardo and de Vasconcelos Maria Lins Carla*, Averrhoa bilimbi L. leaf Aqueous Extract Modulates both Cardiac Contractility and Frequency in the Guinea-pig Atrium by the Activation of Muscarinic Receptors, Letters in Drug Design & Discovery 2018; 15 (11) . https://dx.doi.org/10.2174/1570180815666180125150457